A4929

N,N′-Bis(acryloyl)cystamine

BioReagent, suitable for electrophoresis

• Synonym(s): 2,2′-(Bisacrylamino)diethyl disulfide, BAC, Bis(2-acrylamidoethyl) disulfide
• Empirical Formula (Hill Notation): C₁₀H₁₆N₂O₂S₂
• CAS Number: 60984-57-8
• Molecular Weight: 260.38
• NACRES: NA.25
• PubChem Substance ID: 24890926
• UNSPSC Code: 12352116
• EC Number: 262-546-8
• MDL number: MFCD00036225
• eCl@ss: 32150319
• Form: powder

Properties

• Quality Segment: 200
• product line: BioReagent
• form: powder
• technique(s): protein electrophoresis: suitable
• suitability: suitable for electrophoresis
• storage temp.: −20°C
• SMILES string: C=CC(=O)NCCSSCCNC(=O)C=C
• InChI: 1S/C10H16N2O2S2/c1-3-9(13)11-5-7-15-16-8-6-12-10(14)4-2/h3-4H,1-2,5-8H2,(H,11,13)(H,12,14)
• InChI key: DJVKJGIZQFBFGS-UHFFFAOYSA-N

Description

Application

BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min.

BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): dust mask type N95 (US), Eyeshields, Gloves