A7005

β-Amylase from sweet potato

Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280)

• Synonym(s): 1,4-α-D-Glucan maltohydrolase
• CAS Number: 9000-91-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-566-1
• MDL number: MFCD00081391
• EC Number: 3.2.1.2 (BRENDA, IUBMB)
• Specific activity: ≥750 units/mg protein (E1%/280)
• Biological source: sweet potato
• Concentration: ≥7.0 mg/mL

Properties

• biological source: sweet potato
• Quality Segment: 200
• type: Type I-B
• form: ammonium sulfate suspension
• specific activity: ≥750 units/mg protein (E1%/280)
• greener alternative product score: old score: 57; new score: 1; Find out more about DOZN™ Scoring
• sustainability: Sustainability features
• concentration: ≥7.0 mg/mL
• optimum pH: 4—6
• storage temp.: 2-8°C

Description

General description

β-Amylases belongs to the class of amylolytic enzymes.

Substrate Specificity

Hydrolysis of (1→4)-α-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains

We are committed to bringing you Greener Alternative Products, which adhere to one of the four categories of Greener Alternatives . This product belongs to category of Re-engineered products, showing key improvements in Green Chemistry Principles “Waste Prevention”, “Safer Solvents and Auxiliaries” and “Enhanced Energy Efficiency”. Click here to view its DOZN scorecard.

Application

β -Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. β -Amylase, from Sigma, has been used in various plant studies, such as carbon starvation studies in Populus tremuloides. β-amylase from sweet potato has been used to examine the utility of the enzyme in inhibiting and removing Staphylococcus aureus biofilms. The enzyme has also been used to prepare β-limit dextrin from waxy maize starch.

β-Amylase from sweet potato has been used:

• in the hydrolysis of starch and starch-pectin combinations
• for comparing amylases from different sources in Staphylococcus aureus SH1000 biofilm inhibition and reduction assay
• for selenium speciation in samples

Biochem/physiol Actions

β-Amylases plays a major role in examining enzymatic promiscuity among glycosidases.

β-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. β-amylase from sweet potato is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry.

Physical form

Crystalline suspension in 2.3 M (NH₄)₂SO₄

Other Notes

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 4.8 at 20 °C.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Codes: H334
• Precautionary Statements: P261 - P342 + P311
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• WGK: WGK 3