B6149

5-Bromo-4-chloro-3-indolyl phosphate disodium salt

chromogenic, ≥98% (HPLC), powder

• Synonym(s): 5-BCIP disodium salt, BCIP^®, X-phosphate disodium salt
• Linear Formula: C₈H₄BrClNO₄P · 2Na
• CAS Number: 102185-33-1
• Molecular Weight: 370.43
• UNSPSC Code: 12352204
• PubChem Substance ID: 24891897
• NACRES: NA.83
• MDL number: MFCD00036757
• eCl@ss: 32150319

Properties

• Product Name: 5-Bromo-4-chloro-3-indolyl phosphate disodium salt, ≥98% (HPLC)
• Quality Segment: 200
• assay: ≥98% (HPLC)
• form: powder
• technique(s): protein detection: suitable
• solubility: H₂O: 20 mg/mL, DMF: insoluble
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].[O-]P([O-])(=O)Oc1c[nH]c2ccc(Br)c(Cl)c12
• InChI: 1S/C8H6BrClNO4P.2Na/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2
• InChI key: OAZUOCJOEUNDEK-UHFFFAOYSA-L

Description

General description

Histochemical substrate for alkaline phosphatase.

Application

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.

BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

For the colorimetric detection of alkaline phosphatase-labeled molecules.

5-Bromo-4-chloro-3-indolyl phosphate disodium salt has been used to detect protein-antibody complexes. It has also been used for tissue staining in alkaline phosphatase histochemistry.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)