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GL0010

Golgi Isolation Kit

sufficient for 50 g (tissue)

Synonym(s):

Golgi Kit, Isolation Kit for Golgi

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Size/SKUAvailabilityPrice
1 kit
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$711.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352200

$711.00


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Quality Segment

usage

sufficient for 50 g (tissue)

technique(s)

fractionation: suitable

input

mammalian sample(s)

shipped in

wet ice

storage temp.

2-8°C

General description

The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).

Application

Golgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.

Analysis Note

The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.

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1166732700112033674001CELLYTPN1
description

sufficient for 50 g (tissue)

description

pkg of 1 kit sufficient for 25 purifications

description

sufficient for 50 isolation(s), suitable for RT-PCR, suitable for Northern blotting

description

For plant leaves

technique(s)

fractionation: suitable

technique(s)

-

technique(s)

RT-PCR: suitable, Northern blotting: suitable

technique(s)

ChIP: suitable

usage

sufficient for 50 g (tissue)

usage

-

usage

sufficient for 50 isolation(s)

usage

1 kit sufficient for 30 extractions (20 g of fresh or frozen leaves)

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

200

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

2-8°C

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

wet ice

input

mammalian sample(s)

input

-

input

-

input

plant, fungi and insect sample(s)


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mL
    SDS

Storage Class

10 - Combustible liquids

wgk

WGK 3



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