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[Chemical constituents of the roots of Vaccinium bracteatum].

Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (2012-12-15)
Xiao-Lan Lv, Xi Mai, Hui Guo, Xiao-Ping Lai
ABSTRACT

To study the chemical constituents of the roots of Vaccinium bracteatum. The constituents were separated and purified with chromatographic methods (including silica gel, Sephadex LH-20 and RP-18 column chromatography), and their structures were determined by spectroscopic methods (including MS, 1H-NMR and 13C-NMR). 10 compounds were isolated from the roots of Vaccinium bracteatu and were elucidated as chlorogenic acid (1), pinoresinol (2), ferulic acid (3), kaempferol (4), trans-caffeic acid (5), beta-sitosterol (6), quercetin (7), oleanolic acid (8), apigenin (9) and luteolin (10). Compounds 1 -3 are obtained from this plant for the first time.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
trans-Ferulic acid, ≥99%
Supelco
trans-Ferulic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
trans-Ferulic acid, 99%
Supelco
trans-Ferulic acid, suitable for matrix substance for MALDI-MS, ≥99.0% (HPLC)
Sigma-Aldrich
Chlorogenic acid, ≥95% (titration)
Sigma-Aldrich
Pinoresinol, ≥95.0% (HPLC)
Sigma-Aldrich
Kaempferol, ≥90% (HPLC), powder
Sigma-Aldrich
Kaempferol, ≥97.0% (HPLC)
Chlorogenic acid, primary reference standard
Supelco
Pinoresinol, analytical standard
Supelco
Kaempferol, analytical standard