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Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector.

Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector.

Bio-medical materials and engineering (2014-09-18)

Wenfang Zhao, Kehai Liu, Shunsheng Chen, Man Man Zhu, Hui Lv, Jing Hu, Yuan Mao

ABSTRACT

To solve the contradiction between the cell toxicity and transfection efficiency of polyethylenimine (PEI) derivate in non-viral gene therapy, a novel gene vector, P123-PEI-R18 was synthesized by using biodegradable PEI derivate conjugated with trifunctional peptide RGD-TAT-NLS. The particle size of P123-PEI-R18/DNA was around 100-250 nm. The gene vector could condense DNA at the weight ratio of 2 and protect plasmid DNA from being dissolved in the blood circulation. Importantly, the complexes exhibited lower cell toxicity and higher transfection efficiency contrasted with PEI 25 kDa in vitro. P123-PEI-R18 holds high potential as a safe and efficient gene vector.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Ethyl acetate, suitable for HPLC

Sigma-Aldrich

Benzene, JIS 300, ≥99.5%, suitable for residue analysis

Sigma-Aldrich

Ethanol, ≥99.5%, SAJ super special grade

Sigma-Aldrich

Ethanol, ≥99.5%, suitable for absorption spectrum analysis

Sigma-Aldrich

Ethyl acetate, SAJ first grade, ≥99.0%

Sigma-Aldrich

Ethanol, ≥99.5%, suitable for fluorescence

Sigma-Aldrich

Benzene, JIS special grade, ≥99.5%

Sigma-Aldrich

Ethyl acetate, JIS 300, ≥99.5%, suitable for residue analysis

Sigma-Aldrich

Ethanol, ≥99.5%

Sigma-Aldrich

Benzene, SAJ first grade, ≥99.0%

Sigma-Aldrich

Ethanol, JIS 300, ≥99.5%, suitable for residue analysis

Sigma-Aldrich

Ethanol, JIS 1000, ≥99.5%, suitable for residue analysis

Sigma-Aldrich

Ethyl acetate, JIS special grade, ≥99.5%

Sigma-Aldrich

Ethanol, ≥99.5%, suitable for HPLC

Sigma-Aldrich

Dichloromethane, JIS special grade, ≥99.0%

Sigma-Aldrich

Ethyl acetate, JIS 1000, ≥99.5%, suitable for residue analysis

Sigma-Aldrich

Dichloromethane, SAJ first grade, ≥99.0%

Sigma-Aldrich

Triphosgene, reagent grade, 98%

Sigma-Aldrich

Benzene, anhydrous, 99.8%

Sigma-Aldrich

Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%

Sigma-Aldrich

Ethyl acetate, natural, ≥99%, FCC, FG

Sigma-Aldrich

Ethyl acetate, ≥99%, FCC, FG

Sigma-Aldrich

Ethyl acetate, anhydrous, 99.8%

Sigma-Aldrich

Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%

Sigma-Aldrich

Ethyl alcohol, Pure, 190 proof, meets USP testing specifications

Sigma-Aldrich

N-Hydroxysuccinimide, 98%

Sigma-Aldrich

Dichloromethane, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer

Sigma-Aldrich

Ethyl acetate, ReagentPlus^®, ≥99.8%

Sigma-Aldrich

Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer

Supelco

Dichloromethane, Selectophore^™, ≥99.5%