F4503

Sigma-Aldrich

铁蛋白 来源于马脾脏

Type I, saline solution

CAS号:
MDL编号:
NACRES:
NA.77

质量水平

200

无菌性

sterile-filtered

类型

Type I

形式

saline solution

分子量

H subunit ~21 kDa
L subunit ~19 kDa

颜色

red to brown

痕量阳离子

Cd: ≤1% (as % of ferritin)

储存温度

2-8°C

一般描述

壳蛋白 24 个蛋白质亚基(脱铁铁蛋白)和核心 Fe 3+ 离子,肽 MW 为 440 kDa。回顾铁蛋白样铁储存蛋白超家族,在地球上的生命中几乎无处不在。

应用

来自马脾的铁蛋白已用于:
  • 分枝杆菌生长测定
  • 尺寸排阻色谱
  • 标记巨噬细胞
  • 作为 μ 介子自旋光谱学的参考标准

生化/生理作用

广泛的储铁蛋白在铁代谢中起关键作用。其用作细胞内的铁储备(特别是在脾脏、肝脏、肠粘膜和骨髓中)并参与铁解毒。 研究表明,铁蛋白铁的掺入是由与铁蛋白 H 亚基相关的铁氧化酶活性和与铁蛋白 L 亚基相关的成核中心介导的。 从铁蛋白释放铁在铁依赖性脂质过氧化中具有重要作用,并且可能在体内促成自由基诱导的细胞损伤。因此,通过结合铁,铁蛋白可以起抗氧化剂的作用。

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the concentration of ferritin in solutions of Product F4503, Ferritin from equine spleen?

    The concentration of ferritin is lot specific and can be found on the Certificate of Analysis (CofA) for the specific lot.

  4. Has Product F4503, Ferritin from equine spleen, been cell culture tested?

    The solution is sterile-filtered but has not been tested in cell culture.

  5. Where is iron located in the ferritin molecule of Product F4503, Ferritin from equine spleen?

    Iron is located in the core of the ferritin molecule consisting of 24 subunits. Up to 4500 iron ions can be contained in this core.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Sella Kim et al.
Journal of molecular biology, 428(24 Pt B), 5007-5018 (2016-11-03)
Iron and oxygen chemistry is mediated by iron proteins for many biological functions. Carboxylate-bridged diiron enzymes including ferritin have the common mechanism of oxygen activation via peroxodiferric intermediates. However, the route for iron uptake and the structural identification of intermediates...
Defining the Architecture of the Core Machinery for the Assembly of Fe-S Clusters in Human Mitochondria
Methods in Enzymology, 595, 107-160 (2017)
Italo Moglia et al.
Journal of inorganic biochemistry, 183, 184-190 (2017-12-28)
Ferritin is a globular protein that consists of 24 subunits forming a hollow nanocage structure that naturally stores iron oxyhydroxides. Elimination of iron atoms to obtain the empty protein called apoferritin is the first step to use this organic shell...
The effectiveness of ferritin as a contrast agent for cell tracking MRI in mouse cancer models
Lee CW, et al.
Yonsei Medical Journal, 58(1), 51-58 (2017)
Human-brain ferritin studied by muon spin rotation: a pilot study
Bossoni L, et al.
Journal of Physics. Condensed Matter : An Institute of Physics Journal, 29(41), 415801-415801 (2017)

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