15-105
mTOR phosphorylation Pathway Explorer Antibody MiniPack
This Antibody pack of Anti-mTOR antibodies contains Anti-phospho-mTOR Antibody(Ser2481), Anti-phospho-mTOR Antibody (Thr2446), Anti-phospho-mTOR (Ser2448).
同義詞:
FKBP12-rapamycin complex-associated protein 1, FKBP-rapamycin associated protein, Mammalian target of rapamycin, Rapamycin target protein, rapamycin associated protein FRAP2
跳轉至
生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
affinity isolated antibody
無性繁殖
polyclonal
純化經由
affinity chromatography
製造商／商標名
Upstate®
技術
activity assay: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
phosphorylation (pSer2481/pSer2446/pSer2448)
基因資訊
human ... MTOR(2475)
1 of 4
本產品 | 09-345 | 09-213 | 05-1592 |
|---|---|---|---|
| antibody form affinity isolated antibody | antibody form affinity purified immunoglobulin | antibody form affinity purified immunoglobulin | antibody form purified immunoglobulin |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source mouse |
| Gene Information human ... MTOR(2475) | Gene Information human ... MTOR(2475) | Gene Information human ... MTOR(2475) | Gene Information human ... MTOR(2475) |
| technique(s) activity assay: suitable | technique(s) western blot: suitable | technique(s) western blot: suitable | technique(s) immunocytochemistry: suitable, western blot: suitable |
| shipped in dry ice | shipped in wet ice | shipped in ambient | shipped in wet ice |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
一般說明
Pathway Explorer Antibody MiniPack:
Each Pathway Explorer Antibody Minipack contains three related antibodies as part of a signaling cascade or a combination of total and phosphorylated forms of key signaling targets. Each of the three antibodies are 30% the original pack size. Full size versions of each of the Pathway Explorer antibodies are available for sale individually under the same catalog number with the removal of “SP” off of each one (e.g. 05-591SP can be ordered as 05-591).
mTOR:
mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. Interestingly, mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and GβL (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The complex is activated primarily though the PI3 Kinase/Akt pathway. Upon insulin stimulation, Akt activates mTORC1 by phosphorylating and inhibiting TSC (and possibly other yet discovered targets and/or mTOR itself). This inhibits the upstream small GTPase regulator Rheb (Ras homolog enriched in brain). This inhibits the kinase activity of the mTORC1 complex, thus disabling its ability to phosphorylate its downstream targets such as p70 S6K on Thr389 and 4E-BP1 on Thr229. The other mTOR complex, mTORC2 (mTOR Complex 2), is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Interestingly, unlike mTORC1, the mTORC2 complex appears to not be inhibited by treatment with rapamycin and for this reason is referred to as the rapamycin-insensitive complex. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
*See full size versions for corresponding references.
Each Pathway Explorer Antibody Minipack contains three related antibodies as part of a signaling cascade or a combination of total and phosphorylated forms of key signaling targets. Each of the three antibodies are 30% the original pack size. Full size versions of each of the Pathway Explorer antibodies are available for sale individually under the same catalog number with the removal of “SP” off of each one (e.g. 05-591SP can be ordered as 05-591).
mTOR:
mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. Interestingly, mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and GβL (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The complex is activated primarily though the PI3 Kinase/Akt pathway. Upon insulin stimulation, Akt activates mTORC1 by phosphorylating and inhibiting TSC (and possibly other yet discovered targets and/or mTOR itself). This inhibits the upstream small GTPase regulator Rheb (Ras homolog enriched in brain). This inhibits the kinase activity of the mTORC1 complex, thus disabling its ability to phosphorylate its downstream targets such as p70 S6K on Thr389 and 4E-BP1 on Thr229. The other mTOR complex, mTORC2 (mTOR Complex 2), is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Interestingly, unlike mTORC1, the mTORC2 complex appears to not be inhibited by treatment with rapamycin and for this reason is referred to as the rapamycin-insensitive complex. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
*See full size versions for corresponding references.
免疫原
Epitope: 09-345SP:phosphorylated Thr2446 of mTOR
09-213SP: phosphorylated Ser2448 of mTOR
09-343SP: phosphorylated Ser2481 of mTOR
09-213SP: phosphorylated Ser2448 of mTOR
09-343SP: phosphorylated Ser2481 of mTOR
應用
Pathway Explorer Antibody MiniPack
Research Category
Signaling
Apoptosis & Cancer
Signaling
Apoptosis & Cancer
Research Sub Category
PI3K, Akt, & mTOR Signaling
Glucose/Glycogen Metabolism
Insulin/Energy Signaling
PI3K, Akt, & mTOR Signaling
Glucose/Glycogen Metabolism
Insulin/Energy Signaling
This Antibody pack of Anti-mTOR antibodies contains Anti-phospho-mTOR Antibody(Ser2481), Anti-phospho-mTOR Antibody (Thr2446), Anti-phospho-mTOR (Ser2448).
包裝
Each vial is 30% the size of the parent catalog number
外觀
3 individual tubes each containing antibodies directed against one of the three phosphorylated forms of mTOR (Thr2446, Ser2448, and Ser2481)
其他說明
09-343SP Anti-phospho-mTOR (Ser2481); 30 µL
09-345SP Anti-phospho-mTOR (Thr2446); 30 µL
09-213SP Anti-phospho-mTOR (Ser2448); 60 µL
09-345SP Anti-phospho-mTOR (Thr2446); 30 µL
09-213SP Anti-phospho-mTOR (Ser2448); 60 µL
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
10 - Combustible liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
C L van der Wijden et al.
Nutrition & diabetes, 3, e86-e86 (2013-09-11)
From a public health perspective it is important to know which of the currently used methods to estimate changes in maternal body fat during pregnancy and the year thereafter is the most adequate. To evaluate the concurrent validity between leptin
Arkadiusz D Liśkiewicz et al.
Frontiers in endocrinology, 12, 568197-568197 (2021-03-16)
Obesity cardiomyopathy increases the risk of heart failure and death. Obesity is curable, leading to the restoration of the heart phenotype, but it is not clear if there are any after-effects of obesity present after weight loss. We characterize the
Francesca Bianchini et al.
The British journal of nutrition, 108(12), 2129-2137 (2012-03-07)
Prostate cancer is one of the most common malignancies in men. Epidemiological and experimental studies have revealed that stromal cells of the tumour microenvironment contribute to the development of prostate cancers, while long-chain n-3 PUFA-enriched diets reduce the risk of
Fatemeh Talaei et al.
BMC cell biology, 15, 1-1 (2014-01-08)
Diabetes and particularly high blood glucose levels are implicated in neurodegeneration. One of the hallmarks of neurodegeneration is protein aggregation. We investigated the presence of protein aggregation in the frontal brain of Zucker diabetic fatty (ZDF) rats, an animal model
相關內容
Signaling Product Guide: Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
Active Filters
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務