As per our CofA for # 20-188:
For use in mammalian cell lysis. Dilute one part 10XRIPA Lysis Buffer with nine parts sterile, distilled
water. Stir mixture and refrigerate at least two hours prior to use. Immediately prior to use add the
protease and phosphatase inhibitors for the following final concentrations: 1mM PMSF, 1mM sodium orthovanadate, 1mM sodium fluoride, 1µg/ml aprotinin, 1µg/ml leupeptin, 1µg/ml pepstatin.
Storage and Stability: Stable for 6 months at room temperature from date of shipment.
Store in the dark.
形狀
liquid
包裝
pkg of 100 mL
製造商／商標名
Upstate®
技術
immunoprecipitation (IP): suitable
western blot: suitable
運輸包裝
ambient
1 of 4
本產品 | R0278 | 11814389001 | B6429 |
|---|---|---|---|
| technique(s) immunoprecipitation (IP): suitable, western blot: suitable | technique(s) immunoprecipitation (IP): suitable | technique(s) - | technique(s) Southern blotting: suitable |
| form liquid | form liquid | form solution | form solution |
| packaging pkg of 100 mL | packaging pkg of 50 mL, pkg of 500 mL | packaging pkg of 100 mL | packaging - |
| manufacturer/tradename Upstate® | manufacturer/tradename - | manufacturer/tradename Roche | manufacturer/tradename - |
| shipped in ambient | shipped in - | shipped in - | shipped in - |
一般說明
放射免疫沉淀检测（RIPA）缓冲液有助于贴壁和悬浮培养的哺乳动物细胞进行细胞裂解和蛋白质溶解。此外，这种缓冲液通过减少非特异性蛋白结合相互作用来保持低背景噪音，同时让大多数特异性相互作用得以进行，从而有助于进行相关蛋白-蛋白相互作用研究。
外觀
0.5 M Tris-HCl，pH 7.4，1.5 M NaCl，2.5%脱氧胆酸，10%NP-40，10mM EDTA。
準備報告
室温下保存6个月
其他說明
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
訊號詞
Danger
危險聲明
危險分類
Aquatic Chronic 3 - Eye Dam. 1
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Lena Gamboa et al.
ACS chemical biology, 15(2), 533-542 (2020-01-07)
CRISPR-associated proteins (Cas) are enabling powerful new approaches to control mammalian cell functions, yet the lack of spatially defined, noninvasive modalities limits their use as biological tools. Here, we integrate thermal gene switches with dCas9 complexes to confer remote control
Ya-Hui Chen et al.
Cancer management and research, 12, 4645-4665 (2020-07-02)
Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy with an overall 5-year survival rate of 9.3%, and this malignancy is expected to become the second leading cause of cancer-related death by 2030. Gemcitabine resistance develops within weeks of PDAC
Miles Paszek et al.
Drug metabolism and disposition: the biological fate of chemicals, 48(1), 25-30 (2019-11-11)
Environmental toxicants such as heavy metals from contaminated water or soil and isothiocyanates (ITC) from dietary sources act as pro-oxidants by directly generating reactive oxygen species (ROS) or through depleting cellular antioxidants such as glutathione. Toxicants can alter drug metabolism
Suji Han et al.
Cancers, 11(12) (2019-11-28)
Glioblastoma is a highly aggressive and lethal brain tumor, with limited treatment options. Abnormal activation of the neddylation pathway is observed in glioblastoma, and the NEDD8-activating enzyme (NAE) inhibitor, MLN4924, was previously shown to be effective in glioblastoma cell line
Masahiro Ohtani et al.
Biomedical reports, 12(3), 99-108 (2020-02-12)
Aged garlic extract (AGE) contains various biologically active sulfur-containing amino acids, such as S-allylcysteine (SAC), S-1-propenylcysteine (S1PC) and S-allylmercaptocysteine (SAMC). These amino acids have been demonstrated to lower hypertension, improve atherosclerosis and enhance immunity through their anti-inflammatory and antioxidant activities.
相關內容
Protein Blotting Survival Handbook: Tips and Tricks
Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.
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where is the recommended protocol for this product?
1 answer-
Helpful?
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Can I prepare 10 ml of 1x RIPA buffer and add phosphatase and protease inhibitor then aliquot it to 1 ml Eppendorf tubes and store at -20 for use?
1 answer-
Freeze-thaw cycles may lead to degradation of added phosphatase and protease inhibitors. It is highly recommended to prepare the cocktail just prior to application.
Helpful?
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Can this lysis extract protein from the Nucleus?
1 answer-
RIPA Buffer enables the extraction of cytoplasmic, membrane, and nuclear proteins. Certain proteins are more soluble in RIPA buffer than others.
Helpful?
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How long can 1x RIPA lysis buffer be stored with or without the protease?
1 answer-
This product is stable at room temperature for 6 months from date of shipment. Protease and phosphatase inhibitors should be added immediately prior to use.
Helpful?
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I used the 10X RIPA buffer to lyse my cultured cells. How can I do next? Will it affect the protein quantification by using BCA assay kit? Also, will it affect the SDS-PAGE and Western blot?
1 answer-
Protein samples prepared from cell culture lysates using this 1X RIPA buffer are typically compatible with downstream applications such as BCA assay, SDS-PAGE, and Western blotting. The concentrations of salts and detergents present in the 1X RIPA buffer are relatively low, minimizing interference with these techniques.
Helpful?
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Can we store it a 4 degree, if we want to use it after 6 months?
1 answer-
Studies have not been performed to determine if refrigerated storage will extend the shelf life of this product. The end user would have to determine suitability if used after the recommended 6 months. Note that product R0278, a 1X Ripa Buffer, is stored refrigerated and includes a warranty of 1 year from the date of shipment. Please see the link below to review this product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdfHelpful?
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Someone in lab left the 10X RIPA Buffer in room temperature for overnight. Will it affect the quality of the buffer? Could we continue to use it?
1 answer-
This product does not require refrigeration and is stable at room temperature for up to 6 months.
Helpful?
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Do I used distilled water to dilute to 1X?
1 answer-
The 10X RIPA Lysis Buffer should be diluted with nine parts sterile, distilled water.
Helpful?
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Do I have to dilute to 1X for protein isolation?
1 answer-
Yes, RIPA lysis buffer should be diluted to 1X prior to use.
Helpful?
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