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Merck

R0278

总蛋白提取细胞裂解缓冲液

RIPA Buffer

同義詞:

Radioimmunoprecipitation assay buffer

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MDL號碼:
MFCD02100484
分類程式碼代碼:
12161703
NACRES:
NA.56

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本產品
20-18811814389001T9285
总蛋白提取细胞裂解缓冲液

Sigma-Aldrich

R0278

总蛋白提取细胞裂解缓冲液

RIPA裂解缓冲液，10X 100 mL RIPA Lysis Buffer, 10X for Immunoprecipitation & Western Blotting.

Millipore

20-188

RIPA裂解缓冲液，10X

红细胞裂解液 solution, Roche, pkg of 100 mL, sufficient for 50-500 reactions

Roche

11814389001

红细胞裂解液

Tris-EDTA 缓冲液 100X concentrate for Northern and Southern blotting

Sigma-Aldrich

T9285

Tris-EDTA 缓冲液

technique(s)

immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

200

form

liquid

form

liquid

form

solution

form

liquid

pH

7.8-8.2 (25 °C, 1 ×)

pH

-

pH

-

pH

7.8-8.2 (1 × in water)

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

room temp

color

colorless

color

-

color

-

color

-

一般說明

RIPA（放射免疫沉淀测定）缓冲液是一种无需准备即可使用的即用型溶液。其可最大限度避免非特异性蛋白质结合相互作用，可维持较低的背景，同时支持实现最具特异性的相互作用，从而可以研究相关蛋白质互作。由于大多数抗体和蛋白质抗原不受该缓冲液成分影响，这种成分广泛用于免疫沉淀等应用。
放射免疫沉淀检测缓冲液（RIPA缓冲液）是一种裂解缓冲液，用于从贴壁和悬浮培养的哺乳动物细胞中快速、有效地进行细胞裂解和蛋白质溶解。

應用

RIPA缓冲液已被用作以下实验研究中的细胞裂解试剂：
  • 基于成纤维细胞的外周组织深度表型分型，有助于对零星帕金森病(sPD)进行机制性疾病分层。[1]
  • 验证氧化磷酸化 (OXPHOS)的抑制作用，这种成分可激活人肺上皮细胞的混合谱系激酶域样蛋白(MLKL)依赖性坏死。[2]

RIPA缓冲液能够有效地进行细胞裂解和蛋白质溶解，并同时避免蛋白质的降解以及对蛋白质的免疫反应性和生物活性干扰。 RIPA缓冲液还可在免疫沉淀和分子沉淀检测中造成低的背景。 与EZview™亲和凝胶兼容。

特點和優勢

1 mL RIPA缓冲液可在100 mm培养皿（0.5至5 * 107个细胞）中裂解大多数粘附的哺乳动物细胞系细胞。
将非特异性的蛋白结合互作降低至最低以确保低的背景。

其他說明

含有150 mM NaCl、1.0% IGEPAL® CA-630、0.5%脱氧胆酸钠、0.1% SDS、50 mM Tris, pH 8.0的即用型溶液。

法律資訊

EZview is a trademark of Sigma-Aldrich Co. LLC
IGEPAL is a registered trademark of Solvay

相關產品

產品號碼
描述
訂價

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H319,H412

危險分類

Aquatic Chronic 3 - Eye Irrit. 2

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000504725
0000504726
0000504726
0000504725
0000437799

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Paula Kinsella et al.
Investigational new drugs, 34(2), 159-167 (2016-02-18)
The C-KIT receptor tyrosine kinase is constitutively activated in the majority of gastrointestinal stromal tumours (GIST). Imatinib (IM) a selective inhibitor of C-KIT, is indicated for the treatment of KIT-positive unresectable and/or metastatic GIST, and has tripled the survival time
Zuzana Keckesova et al.
Nature, 543(7647), 681-686 (2017-03-23)
Post-mitotic, differentiated cells exhibit a variety of characteristics that contrast with those of actively growing neoplastic cells, such as the expression of cell-cycle inhibitors and differentiation factors. We hypothesized that the gene expression profiles of these differentiated cells could reveal
Impaired oxidative phosphorylation regulates necroptosis in human lung epithelial cells
Koo MJ, et al.
Biochemical and Biophysical Research Communications, 464(3), 875-880 (2015)
Elham Abbasloo et al.
Neuropeptides, 60, 7-12 (2016-08-21)
Pain represents a major contributing factor to the individual's quality of life. Although pain killers as opioids, endogenous or exogenous peptides can decrease pain perception, the chronic use of them leads to antinociceptive tolerance. It has been demonstrated that neuropeptide
Hartmut Jahns et al.
Nature communications, 6, 6317-6317 (2015-03-07)
An established means of improving the pharmacokinetics properties of oligoribonucleotides (ORNs) is to exchange their phosphodiester linkages for phosphorothioates (PSs). However, this strategy has not been pursued for small interfering RNAs (siRNAs), possibly because of sporadic reports that PS siRNAs
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Product Information Sheet - R0278

Instructions

CelLytic Protein Extraction Reagents
GC5, GC10, and Thunderbolt Competent Cells
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Questions

1–10 of 12 Questions  

  1. we bought a bottle of RIPA buffer and want to make alliquots of this bottle. Can we store the alliquots of the buffer by -20? Or is it better to keep them between 2°C-8°C ?

    1 answer

    1. It is recommended to store the product at 2–8C. Studies have not been performed on aliquots and to determine if refrigerated storage will extend the shelf life of this product. We would recommend searching the general research literature or contacting researchers who routinely use this product to see if solution stability information is available. If a recommended storage is not given in the literature, it is usually best to make solutions fresh for use. If you decide to store the aliquots at -20°C, we would recommend validating activity of the supplemented buffer after freezing and avoid any thawing/freezing cycle. Please refer to this technical bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf

      Helpful?

  2. Is this RIPA lysis buffer capable of extracting nuclear protein in mammalian cells?

    1 answer

    1. Yes, RIPA buffer typically lyses cell nuclei. RIPA (Radio-Immunoprecipitation Assay) Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. For more information, please see the Technical Bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf

      Helpful?

  3. Can I use R0278 for human protein extract for lymphocytes?

    1 answer

    1. This product has not been tested for protein extract from human lymphocytes. References using RIPA buffer with human lymphocytes can be found here:
      https://www.sciencedirect.com/science/article/pii/S0021925818639675
      https://www.sciencedirect.com/science/article/pii/S000887491530037X?via%3Dihub

      Helpful?

  4. Can I use the Ripa Buffer to extract yeast protein and make the Bradford assay on it?

    1 answer

    1. RIPA (Radio-Immunoprecipitation Assay) Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. For more information, please see the Technical Bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf

      Helpful?

  5. How long can the RIPA buffer be stored in 4C? I stored it for almost 6 years, can I still use it?

    1 answer

    1. Regarding stability in the recommended temperature of 2-8 °C, this product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

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  6. Can this be used for Western blot experiments?

    1 answer

    1. Yes, this product would be suitable for western blotting.

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  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. How does Product R0278, RIPA buffer, compare to Product No. C2978 (CelLytic M)?

    1 answer

    1. RIPA buffer contains SDS, which is considered to be a harsh detergent; it can denature many proteins. C2978 is a buffered solution (pH 7.6) that contains a mild detergent; it is non-denaturing for most proteins.  The exact formulation of C2978, however, is proprietary.

      Helpful?

  9. I left the bottle of Product R0278, RIPA buffer, on my bench for about a week. Can I still use it?

    1 answer

    1. Yes, you should be able to use this product.  Although we recommend storage in the refrigerator at 2-8°C for long-term storage, Product No. R0278 may be kept at ambient temperature (our normal shipping condition) for a short time (approximately 2 weeks).

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  10. Can Product R0278, RIPA buffer, be used on tissue samples, or only on cultured cells?

    1 answer

    1. R0278 can be used on tissue, if it is well homogenized.

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