33-047
PI 3-激酶3步式HTRF®检测
The PI 3-Kinase 3-Step HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format.
同義詞:
3步式HTRF检测, PI 3-激酶检测
跳轉至
品質等級
物種活性
human, mouse
製造商／商標名
HTRF®
Upstate®
技術
activity assay: suitable (kinase)
NCBI登錄號
UniProt登錄號
檢測方法
fluorometric (HTRF)
運輸包裝
dry ice
基因資訊
human ... PIK3CA(5290)
1 of 4
本產品 | 33-036 | 14-782-M | SRP0233 |
|---|---|---|---|
| technique(s) activity assay: suitable (kinase) | technique(s) activity assay: suitable (kinase) | technique(s) activity assay: suitable (kinase) | technique(s) activity assay: suitable |
| species reactivity human, mouse | species reactivity mouse, human | species reactivity - | species reactivity - |
| manufacturer/tradename HTRF® | manufacturer/tradename HTRF®, Upstate® | manufacturer/tradename Upstate® | manufacturer/tradename Sigma-Aldrich |
| NCBI accession no. | NCBI accession no. | NCBI accession no. | NCBI accession no. |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| detection method fluorometric (HTRF) | detection method fluorometric (HTRF) | detection method - | detection method - |
一般說明
磷脂酰肌醇-4,5-二磷酸3激酶催化亚基α(PIK3CA)基因定位于人类染色体3q26.32。该基因编码IA类PI3激酶的催化亚基p110α。PI3K信号通路调节多种细胞活动和特性，包括增殖、存活、凋亡、运动和形态。PIK3CA基因的突变或过表达与各种人类实体肿瘤的发展有关，包括头颈癌或咽喉癌和卵巢癌。
應用
PI 3-激酶3步式HTRF®检测已用于测定磷脂酰肌醇3-激酶(PI3K)δ抑制剂（如MSD-496486311、MSD126796721、ideelalisib和duvelisib）对PI3K α、β、γ和δ异构体的体外抑制效力。
生化／生理作用
靶标亚家族：PI3K/PI4K
包裝
试剂盒容量：10,000孔
準備報告
在-70°C下可保存12个月
其他說明
4X反应缓冲液
PIP2（底物；1mM储备液）
DM A反应缓冲液
DM B反应缓冲液
DM C反应缓冲液
BPIP3
终止试剂
PIP2（底物；1mM储备液）
DM A反应缓冲液
DM B反应缓冲液
DM C反应缓冲液
BPIP3
终止试剂
研究类别
细胞凋亡 & 癌症
研究子类别
肿瘤学
细胞凋亡 & 癌症
研究子类别
肿瘤学
法律資訊
HTRF is a registered trademark of Cis Bio International
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Robbie L McLeod et al.
The Journal of pharmacology and experimental therapeutics, 369(2), 223-233 (2019-02-26)
We leveraged a clinical pharmacokinetic (PK)/pharmacodynamics (PD)/efficacy relationship established with an oral phosphatidylinositol 3-kinase (PI3K)δ inhibitor (Idelalisib) in a nasal allergen challenge study to determine whether a comparable PK/PD/efficacy relationship with PI3Kδ inhibitors was observed in preclinical respiratory models of
Andreas G Bader et al.
Nature reviews. Cancer, 5(12), 921-929 (2005-12-13)
There have long been indications of a role for PI3K (phosphatidylinositol 3-kinase) in cancer pathogenesis. Experimental data document a requirement for deregulation of both transcription and translation in PI3K-mediated oncogenic transformation. The recent discoveries of cancer-specific mutations in PIK3CA, the
Wanglong Qiu et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 12(5), 1441-1446 (2006-03-15)
Recent studies have reported high frequencies of somatic mutations in the phosphoinositide-3-kinase catalytic alpha (PIK3CA) gene in several human solid tumors. Although gene amplifications of PIK3CA have been reported in head and neck squamous cell carcinoma (HNSCC), small mutation of
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