33-036

PI 3-Kinase HTRF^® Assay; 10,000 wells

Name: PI 3-Kinase HTRF<SUP>®</SUP> Assay; 10,000 wells
Brand: MM

The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 10,000 wells.

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分類程式碼代碼:

12161503

eCl@ss:

32161000

NACRES:

NA.84

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品質等級

100

物種活性

mouse, human

製造商／商標名

HTRF^®
Upstate^®

技術

activity assay: suitable (kinase)

NCBI登錄號

NM_006218.2

UniProt登錄號

P42336

檢測方法

fluorometric (HTRF)

運輸包裝

dry ice

基因資訊

human ... PIK3CA(5290)

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本產品	33-017	33-016	33-047
Sigma-Aldrich 33-036 PI 3-Kinase HTRF^® Assay; 10,000 wells	Sigma-Aldrich 33-017 HTRF^®磷脂酰肌醇3激酶活性测定试剂盒，1920 孔	Sigma-Aldrich 33-016 PI3-激酶 HTRF ^®含量测定；384 孔	Sigma-Aldrich 33-047 PI 3-激酶3步式HTRF^®检测
technique(s) activity assay: suitable (kinase)	technique(s) activity assay: suitable (kinase)	technique(s) activity assay: suitable (kinase)	technique(s) activity assay: suitable (kinase)
species reactivity mouse, human	species reactivity mouse, human	species reactivity human, mouse	species reactivity human, mouse
manufacturer/tradename HTRF^®	manufacturer/tradename HTRF^®, Upstate^®	manufacturer/tradename HTRF^®, Upstate^®	manufacturer/tradename HTRF^®, Upstate^®
NCBI accession no. NM_006218.2	NCBI accession no. NM_006218.2	NCBI accession no. NM_006218.2	NCBI accession no. NM_006218.2
UniProt accession no. P42336	UniProt accession no. P42336	UniProt accession no. P42336	UniProt accession no. P42336
detection method fluorometric (HTRF)	detection method fluorometric (HTRF)	detection method fluorometric (HTRF)	detection method fluorometric (HTRF)

一般說明

Phosphoinositide (PI) 3-kinase (PI3K) is a heterodimeric lipid kinase containing regulatory and catalytic subunits. It catalyzes the phosphorylation at the 3-position hydroxyl group of the inositol ring of phosphatidylinositol (PtdIns). PI3K is implicated in various cellular functions such as cell growth, proliferation, motility, differentiation, survival, apoptosis, cytoskeletal rearrangement, and intracellular trafficking. It also plays a vital role in glucose uptake triggered by insulin.

應用

PI 3-Kinase HTRF^® Assay; 10,000 wells has been used for measuring the enzymatic activity of the class I phosphoinositide (PI) 3-kinase isoforms by a time-resolved fluorescence resonance energy transfer (TR-FRET) assay.

生化／生理作用

Target Sub-Family: PI3K/PI4K

包裝

Kit capacity: 10,000 wells

準備報告

12 months at -70°C

其他說明

4X Reaction Buffer

PIP2 (Substrate; 1mM stock)

Stop Reagent A

Stop Reagent B

DM A Detection Reagent

DM B Detection Reagent

DM C Detection Reagent

法律資訊

HTRF is a registered trademark of Cis Bio International

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

分析證明 (COA)

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PI 3-Kinase HTRF^® Assay; 10,000 wells

The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 10,000 wells.