The kit is compatible with mouse samples for STAT1, 3, and 5 only. However, it is a nonconfigurable kit, so the full panel would need to be purchased.
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本產品 | 48-623MAG | 48-621MAG | 48-690MAG |
|---|---|---|---|
| species reactivity human | species reactivity human, mouse | species reactivity mouse, human, rat | species reactivity human |
| manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex® xMAP®) | detection method fluorometric (Luminex® xMAP®) | detection method fluorometric (Luminex xMAP) |
一般說明
The STAT (Signal Transducers and Activators of Transcription) family, consisting of seven mammalian members, regulates many aspects of growth, survival and differentiation in cells. The extracellular binding of cytokines, hormones, growth factors and neurotransmitters to receptors induces phosphorylation of the intracellular Janus kinases (JAKs) that, in turn, phosphorylate STAT proteins at conserved tyrosine residues, activating SH2-mediated dimerization followed by translocation to the nucleus. STAT dimers then bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes.
應用
- An overnight (4°C) incubation is recommended for best results.
- This assay requires 25 μL diluted cell lysate per well.
- This kit must be run using Assay Buffer 1 (provided).
- 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).
Research Category
Signaling|Apoptosis & Cancer
Signaling|Apoptosis & Cancer
The analytes available for this multiplex kit are: STAT1 (Tyr701), STAT2 (Tyr690), STAT3 (Tyr705), STAT5A/B (Tyr694/699), STAT6 (Tyr641).
Used to detect/quantify:STAT1 (Tyr701)|STAT2 (Tyr690)|STAT3 (Tyr705)|STAT5A/B (Tyr694/699)|STAT6 (Tyr641)
生化／生理作用
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
包裝
96-well plate
準備報告
Recommended storage for kit components is 2 - 8°C.
法律資訊
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
訊號詞
Danger
危險分類
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
相關內容
See how multiplexing the inflammation signaling pathway with MILLIPLEX® inflammation assays or cell signaling assays can help researchers bridge the gap between immunology and cell signaling, including investigating T cell signaling, Th Cell differentiation, inflammatory response signaling, and sepsis signaling.
MILLIPLEX®細胞訊號傳導分析儀有助於檢測細胞通路中的磷酸化蛋白和總蛋白。
MILLIPLEX® 分析法橋樑免疫學和細胞信號傳導，用於炎症研究，包括 T 細胞信號傳導和敗血症信號傳導。
Uncover how cells communicate with MILLIPLEX® cell signaling multiplex assays. Multiplexing with cell signaling phosphoprotein assays based on Luminex® xMAP® technology helps researchers measure phosphoproteins and total proteins within the same or different pathways from a single sample.
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Is there a mouse kit similar to 48-610MAG, which can detect mouse phospho-STAT 1, 3, and 5 proteins?
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