48-660MAG

MILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay

• 同義詞: Cell Signaling Kit, MAPK/SAPK 10-Plex Kit
• 分類程式碼代碼: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

屬性

• 物種活性: human
• 品質等級: 200
• 製造商／商標名: Milliplex^®
• 技術: multiplexing: suitable
• assay range: inter-assay cv: 4-13%; intra-assay cv: 3-10%
• 檢測方法: fluorometric (Luminex xMAP)
• 儲存溫度: 2-8°C

描述

一般說明

Cells respond to their environment in various ways through intracellular signaling. Signaling through Receptor Tyrosine kinases (RTKs) often promotes increased metabolism and cell growth. Activation of ERK/MAP kinase is one of the key serine/threonine kinases activated via RTK signaling, promoting increased activity of p70 S6 kinase, MSK1, STATs, CREB, and many other signaling intermediates. Other signaling pathways induced via stress (ex. heat shock/arsenite treatment) or death receptors (ex. TNFα) promote p38, JNK, and p53 signaling pathways.

應用

• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 μL diluted cell lysate.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.

Research Category
Signaling

Used to detect/quantify:ATF2 (Thr71)|Erk (Thr185/Tyr187)|HSP27 (Ser78)|JNK (Thr183/Tyr185)|c-Jun (Ser73)|MEK1 (Ser222)|MSK1 (Ser212)|p38 (Thr180/Tyr182)|p53 (Ser15)|STAT1 (Tyr701)

生化／生理作用

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

法律資訊

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

安全資訊

• 象形圖: GHS05,GHS07,GHS09
• 訊號詞: Danger
• 危險聲明: H302,H315,H318,H410
• 防範說明: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• 危險分類: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• 儲存類別代碼: 10 - Combustible liquids