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Merck

70622-M

Tuner Competent Cells - Novagen

Escherichia coli, rod shaped

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分類程式碼代碼:
41106202

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產品名稱

Tuner Competent Cells - Novagen, Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.

生物源

Escherichia coli

品質等級

製造商/商標名

Novagen®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

增長模式

adherent or suspension

形態學

rod shaped

技術

microbiological culture: suitable

細胞轉化

transformation efficiency: >2×106 cfu/μg

運輸包裝

dry ice

儲存溫度

−70°C

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本產品
706247062371404-M
technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

growth mode

adherent or suspension

growth mode

adherent or suspension

growth mode

adherent or suspension

growth mode

adherent or suspension

biological source

Escherichia coli

biological source

Escherichia coli

biological source

Escherichia coli

biological source

Escherichia coli

morphology

rod shaped

morphology

rod shaped

morphology

rod shaped

morphology

rod shaped

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice, wet ice

shipped in

dry ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

一般說明

Genotype: FompT hsdSB (rB mB) gal dcm lacY1

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Tuner strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture. The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction. By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors. Lower-level expression may enhance the solubility and activity of difficult target proteins.
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.

其他說明

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlTuner Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

法律資訊

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


分析證明 (COA)

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Kalle Sipilä et al.
Developmental cell, 57(12), 1453-1465 (2022-06-08)
Stem cell renewal and differentiation are regulated by interactions with the niche. Although multiple cell populations have been identified in distinct anatomical compartments, little is known about niche-specific molecular factors. Using skin as a model system and combining single-cell RNA-seq

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