MAB377C3

Anti-NeuN Antibody, clone A60, Cy3 Conjugate

clone A60, from mouse, CY3 conjugate

• 同義詞: NEUronal Nuclei, Neuronal Nuclei
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 共軛: CY3 conjugate
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: A60, monoclonal
• 物種活性: rat
• 物種活性（以同源性預測）: mouse (based on 100% sequence homology)
• 技術: immunocytochemistry: suitable
• 同型: IgG1
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Rbfox3(52897) ; rat ... Rbfox3(287847)

描述

一般說明

NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).

The unconjugated parent antibody (Cat. No. MAB377) has an observed molecular weight in the 46-48 kDa range and possibly another band at approximately 66 kDa

免疫原

Purified cell nuclei from mouse brain.

應用

Detect NeuN using this Anti-NeuN Antibody, clone A60, Cy3 Conjugate validated for use in IC.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

生化／生理作用

NeuN (or Neuronal Nuclei) reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia.

外觀

Protein A purified

Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

準備報告

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

分析報告

Control
Rat E18 primary cortex neurons.

Evaluated by Immunocytochemistry in rat E18 primary cortex cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 primary cortex cells.

其他說明

Unconjugated Anti-NeuN has been shown to work with multiple species including Human, Mouse, Ferret, Chicken, and Avian.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable