生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified immunoglobulin
無性繁殖
10C2, monoclonal
物種活性
human
應無反應活性
rat, mouse
包裝
antibody small pack of 25 μg
製造商／商標名
Chemicon®
技術
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
輸入
sample type epithelial cells
sample type neural stem cell(s)
同型
IgG1
NCBI登錄號
UniProt登錄號
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
相關類別
1 of 4
本產品 | MAB5326C3 | FCMAB313PE | MAB353B |
|---|---|---|---|
| species reactivity human | species reactivity human | species reactivity human | species reactivity mouse, rat |
| antibody form purified immunoglobulin | antibody form purified antibody | antibody form purified antibody | antibody form purified immunoglobulin |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| clone 10C2, monoclonal | clone 10C2, monoclonal | clone 10C2, monoclonal | clone rat-401, monoclonal |
| technique(s) immunocytochemistry: suitable, western blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | technique(s) immunocytochemistry: suitable | technique(s) flow cytometry: suitable | technique(s) immunocytochemistry: suitable, immunohistochemistry: suitable |
| shipped in ambient | shipped in wet ice | shipped in wet ice | shipped in wet ice |
一般說明
免疫原
應用
WI-38（ATCC＃CCL75）可用作阳性对照。U251细胞系也可作为阳性对照。
免疫细胞化学：以1：200的稀释度用于固定在4％多聚甲醛中并用0.02％Triton渗透的细胞上
免疫组织化学：低聚甲醛是首选的固定剂：以1：200的稀释度用于福尔马林固定、石蜡包埋的组织切片。仅封闭过程中使用0.02％triton；稀释缓冲液含0.1％BSA；室温孵育1-2小时。建议将人胎脑作为阳性对照。(CA Messam et al, Develop.Brain Res, 134:87-92, 2002; Gu, et al., Brain Res. 943:174-180, 2002).细胞质中的丝状染色
最佳工作稀释液必须由最终用户进行确定。
神经干细胞
干细胞研究
生化／生理作用
外觀
準備報告
在运输过程中，少量产品有时会夹在产品瓶的密封中。对于体积为200μL或更小的产品，我们建议在小瓶的硬质表面上轻轻敲打，或在台式离心机中短暂离心以收集容器′盖中的所有液体。
分析報告
阳性对照：WI-38（ATCC＃CCL75）或U251细胞系
其他說明
法律資訊
免責聲明
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
文章
Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.
用於產生神經幹細胞、神經元和膠質細胞類型的人類 iPSC 神經分化培養基和方案。
ReNcell 神經祖細胞是永生化的人類神經幹細胞系，可以分化成神經元、星形細胞和少突細胞。
ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.
條款
使用 ReNcell 人類神經幹細胞系製造阿爾茨海默氏症 3D NSC 模型的幹細胞培養方案。
神經幹細胞分離、擴展、分化和表徵等分步培養方案。
A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
相關內容
As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.
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