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Merck

A7906

牛血清白蛋白 来源于牛血清

heat shock fraction, pH 7, ≥98%

同義詞:

BSA, 牛白蛋白, 牛血清白蛋白

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CAS 編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.27

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生物源

bovine

品質等級

化驗

≥98%

形狀

lyophilized powder

分子量

~66 kDa

純化經由

heat shock fractionation

包裝

poly bottle of

來源

USA origin

技術

blood typing: suitable
microbiological culture: suitable

雜質

≤5.0% Loss on drying

損耗

≤5%

pH值

7

溶解度

water: soluble (40 mg/ml)

UniProt登錄號

異物活動

BT virus, none detected
VSV virus, none detected

儲存溫度

2-8°C

基因資訊

bovine ... ALB(280717)

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Show Differences

1 of 4

本產品
A9647A8022A3059
origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

biological source

bovine

biological source

bovine

biological source

bovine

biological source

bovine

assay

≥98%

assay

≥98%

assay

≥96%

assay

≥98%

technique(s)

blood typing: suitable, microbiological culture: suitable

technique(s)

immunocytochemistry: suitable

technique(s)

ELISA: suitable, blocking: suitable, electrophoresis: suitable, western blot: suitable

technique(s)

ELISA: suitable, western blot: suitable

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

300

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

應用

牛血清白蛋白已用于:
  • 作为 PCR(聚合酶链反应)混合物的成分[1]
  • 分离的皮层神经元的制备[2]
  • 免疫荧光标记[2]
  • 作为用于牛组织消化的无 Ca2+ 盐水(含有胶原酶、透明质酸酶和 DNA 酶)的成分[3]

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

準備報告

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克分级制备

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

Lot/Batch Number

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已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

A study of the bovine adrenal chromaffin nicotinic receptor using patch clamp and concentration-jump techniques.
Maconochie DJ and Knight DE
The Journal of Physiology, 454, 129-153 (1992)
New tetranucleotide microsatellites for fine-scale discrimination among endangered Chinook salmon (Oncorhynchus tshawytscha).
Greig C et al
Molecular Ecology Notes, 3(3), 376-379 (2003)
The slice overlay assay: a versatile tool to study the influence of extracellular signals on neuronal development.
Polleux F and Ghosh A
Science's STKE : Signal Transduction Knowledge Environment, 2002(136), pl9-pl9 (2002)
V Templier et al.
Scientific reports, 7(1), 9457-9457 (2017-08-27)
Bloodstream bacterial infections are life-threatening conditions necessitating prompt medical care. Rapid pathogen identification is essential for early setting of the best anti-infectious therapy. However, the bacterial load in blood samples from patients with bacteremia is too low and under the
Anna Sintsova et al.
PLoS pathogens, 10(9), e1004341-e1004341 (2014-09-05)
An overwhelming neutrophil-driven response causes both acute symptoms and the lasting sequelae that result from infection with Neisseria gonorrhoeae. Neutrophils undergo an aggressive opsonin-independent response to N. gonorrhoeae, driven by the innate decoy receptor CEACAM3. CEACAM3 is exclusively expressed by

Questions

1–9 of 9 Questions  
  1. How long is BSA (A7906) viable at room temperature? 200g was left on benchtop overnight (Approximately 22 hours), will it still work?

    1 answer
    1. This item is stored refrigerated at 2-8°C. However, the product is shipped at ambient temperature. Shipping routes are configured to minimize transit time.
      Refrigerated products that ship at ambient temperature are considered stable up to 2 weeks for transit time.

      Helpful?

  2. Is the product A7906, Albumin from bovine serum (BSA), fatty acid-free?

    1 answer
    1. Helpful?

  3. After reconstitution in PBS, for how long is the protein stable in solution if stored at 4°C or -20*C?

    1 answer
    1. Prepared BSA solution products also available for purchase. These products have a shelf life ranging from 18 months to 2 years. Many are assigned a restest schedule with no firm expiration date. These products are prepared in saline, DPBS, or Tris. These solutions are filtered products and offered in a range of concentrations. The storage recommendation for these products is 2 - 8 deg. C. In addition, BSA is often used as a carrier protein for less stable compounds that are stored frozen. While there is no established shelf life for a BSA solution stored frozen, it is quite stable. See the link below for a sample Certificate of Analysis for one of the BSA solutions.

      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A9576-50MLSLCL8813.pdf

      Helpful?

  4. Does this BSA have bovine immunoglobulins?

    1 answer
    1. This product does contain bovine immunoglobulins. BSA products that do not contain immunoglobulins will state "globulin-free" or "essentially globulin-free" on the product detail page. Product A3156 is an example of an immunoglobulin free BSA product.

      Helpful?

  5. Is Product A7906, Albumin from bovine serum (BSA), protease-free?

    1 answer
    1. We have not tested A7906 in quality control to be protease-free. Other BSA products to suggest that are protease-free would be A3059 or B6917.

      Helpful?

  6. Is Product A7906, Albumin from bovine serum (BSA), tested for insulin levels?

    1 answer
    1. A7906 is not tested in quality control for insulin levels. One BSA that is indicated as "suitable for insulin RIA procedures" is A7888.

      Helpful?

  7. Is Product A7906, Albumin from bovine serum (BSA), suitable for use as a blocking agent in ELISA?

    1 answer
    1. We have not tested the use of A7906 in ELISA. Because the remainder of the product is mostly globulins, we would not suggest A7906 for ELISA use.

      Helpful?

  8. Can Product A7906, Albumin from bovine serum (BSA), be used in immunohistochemistry?

    1 answer
    1. We have not ourselves tested A7906 for use in immunohistochemistry. However, a literature search can turn up publications that cite this use, such as this paper: Surendran, K., et al., Wnt-Dependent Beta-Catenin Signaling Is Activated after Unilateral Ureteral Obstruction, and Recombinant Secreted Frizzled-Related Protein 4 Alters the Progression of Renal Fibrosis. J. Am. Soc. Nephrol., Vol. 16, pp. 2373-2384 (2005).

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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