If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
P1379
TWEEN ® 20
viscous liquid
同義詞:
聚氧乙烯山梨糖醇酐单月桂酸酯, 聚氧乙烯脱水山梨醇单月桂酸酯
跳轉至
描述
non-ionic
品質等級
化驗
>40% (GC)
形狀
viscous liquid
分子量
~1228
成份
lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
濃度
≥40.0% (GC)
技術
DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable
雜質
≤3.0% water
折射率
n20/D 1.468 (lit.)
CMC
0.06 mM (20-25°C)
酸值
≤2.2 mg/g
羥值
96‑108 mg/g
轉變溫度
cloud point 76 °C
密度
1.095 g/mL at 25 °C (lit.)
HLB
16.7
適合性
complies for IR spectroscopy
SMILES 字串
CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
InChI
1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
InChI 密鑰
HMFKFHLTUCJZJO-UHFFFAOYSA-N
尋找類似的產品？ 前往 產品比較指南
1 of 4
本產品 | P9416 | P2287 | 93773 |
|---|---|---|---|
| technique(s) DNA extraction: suitable, cell culture | mammalian: suitable, RNA extraction: suitable, protein purification: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) DNA extraction: suitable, RNA extraction: suitable, cell culture | mammalian: suitable, protein purification: suitable |
| suitability complies for IR spectroscopy | suitability - | suitability - | suitability - |
| assay >40% (GC) | assay >40% (GC) | assay - | assay - |
| form viscous liquid | form viscous liquid | form viscous liquid | form viscous liquid |
| HLB 16.7 | HLB - | HLB 16.7 | HLB - |
| impurities ≤3.0% water | impurities RNAse, none detected, endonuclease, none detected, exonuclease, none detected | impurities - | impurities - |
一般說明
表面活性剂同时具有亲水和疏水成分，可以分为两大类：在水中电离的离子型（阴离子、阳离子或两性）和不电离的非离子型 。所述产品为非离子表面活性剂，可用于各种应用，如乳化、分散、增溶和去污剂。此外，它可用作膜蛋白的增溶剂。
應用
通常0.05%浓度的TWEEN 20在基于膜的免疫检测中被用作封闭剂。0.005至0.5%的TWEEN 20可用于裂解哺乳动物细胞。
特點和優勢
- 用于生命科学和生物化学研究的多功能表面活性剂
- 良好的增溶、分散和乳化剂
- 适用于DNA & RNA 提取、哺乳动物细胞培养和蛋白质纯化
包裝
每个试剂盒包含3 x 100ML样品，每份样品都有唯一的制造商批号。
準備報告
法律資訊
同類產品
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
527.0 °F - Pensky-Martens closed cup
閃點（°C）
275 °C - Pensky-Martens closed cup
個人防護裝備
Eyeshields, Gloves
文章
可用的螢光原位雜交 (FISH) 程序、試劑和設備。
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
條款
本頁顯示如何使用 Cytiva 的產品溶解膜蛋白。
This page shows how to solubilize membrane proteins with products from Cytiva.
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
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What is the storage temperature of Tween 20?
1 answer-
This product may be stored at room temperature.
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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What is the minimum dilution ratio of Tween20 in Exosome incubating buffer based on CMC level?
1 answer-
This product is not tested for any specific application. The critical micelle concentration of TWEEN 20 has been reported as 60 milligrams per liter. Please see the link below to review a publication that may be helpful:
https://open.clemson.edu/cgi/viewcontent.cgi?article=5274&context=all_thesesHelpful?
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Please provide the information on product container and material for P1379-500ML TWEEN® 20
1 answer-
This item is packaged in a 500 ML HDPE Bottle with a red PP cap w/ PTFE liner.
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You state 0.06 mM as the CMC for this product. What is the stock concentration (in Molar) for this product? Alternatively what is the % to reach that 0.06 mM / CMC concentration? Thank you
1 answer-
The critical micelle concentration (CMC) reported on the product page is a value reported from the literature. This product is a neat or pure liquid. The base molecular weight of this material is 1,225 daltons and the density is 1.095 g/mL. However, this is based on the assumption of 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid. Please see the link below to review the general Tween 20 datasheet, note that the CMC value listed is 60 mg/L.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/128/958/p9416pis.pdfHelpful?
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What is the grade/agency of Tween 20?
1 answer-
This product is a research grade TWEEN 20.
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What is the expiry date once opened?
1 answer-
Long-term stability studies have not been performed, however, providing that the product is stored and handled properly, the expected shelf life is not expected to change. Factors such as improper closure and cross-contamination would certainly impact the overall stability and shelf life.
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The data sheet for my lot says this is 50% pure. Does that mean it is 50% tween 20?
1 answer-
The 50% is referring to the concentration of lauric acid which occurs in the highest concentration. Fatty acid constituents of this product are determined by transesterification to yield fatty acid methyl esters, which are identified by gas chromatography. Fatty acids that are typically present in the offered Tween products are not a quantitative measurement. These include myristic, palmitic, and stearic acid, for the most part. While the Tween is considered a 'pure' product, the molecular weight is calculated on the assumption of 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid.
Helpful?
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The appearance of Product P1379, Tween® 20, is clear dark yellow. Is this OK?
1 answer-
Yes. The specifications for P1379 are "clear yellow to yellow green viscous liquid".
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