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Merck

A3221

碘乙酰胺

Single use vial of 56 mg

Iodoacetamide
1 of 2 reviewers received a sample product or took part in a promotion

同義詞:

IAA

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關於此項目

線性公式:
ICH2CONH2
CAS 編號:
分子量::
184.96
UNSPSC Code:
12352111
NACRES:
NA.56
PubChem Substance ID:
EC Number:
205-630-1
Beilstein/REAXYS Number:
1739080
MDL number:

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產品名稱

碘乙酰胺, Single use vial of 56 mg

InChI

1S/C2H4INO/c3-1-2(4)5/h1H2,(H2,4,5)

InChI key

PGLTVOMIXTUURA-UHFFFAOYSA-N

SMILES string

NC(=O)CI

biological source

synthetic (organic)

assay

≥99% (HPLC)

form

powder

packaging

vial of 56 mg (Single use)

storage condition

(Tightly closed. Dry. Keep in a well-ventilated place. Keep locked up or in an area accessible
only to qualified or authorized persons.)

color

white to off-white

mp

92-95 °C (lit.)

solubility

H2O: soluble 50 mg/mL, clear, colorless to faintly yellow

suitability

suitable for LC-MS

application(s)

microbiology

storage temp.

2-8°C

Quality Level

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1 of 4

本產品
I5161I6125I1149
assay

≥99% (HPLC)

assay

≥99% (NMR)

assay

≥99% (NMR)

assay

≥99% (NMR)

biological source

synthetic (organic)

biological source

synthetic

biological source

synthetic (organic)

biological source

synthetic (organic)

Quality Level

200

Quality Level

400

Quality Level

300

Quality Level

300

form

powder

form

powder

form

crystalline

form

powder

solubility

H2O: soluble 50 mg/mL, clear, colorless to faintly yellow

solubility

H2O: 0.5 M, clear, colorless

solubility

H2O: soluble 0.5 M, clear, colorless

solubility

H2O: 0.5 M, clear, colorless

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

碘乙酰胺已用于:
  • 防止蛋白质样品发生酶促二硫键还原反应[1]
  • 在细胞生物学研究中抑制甘油醛-3-磷酸脱氢酶,有效阻断ATP生产[1]

Biochem/physiol Actions

碘乙酰胺在肽测序中胱氨酸残基的烷基化试剂。它是活性位点上含有胱氨酸的酶的的一种不可逆抑制剂。它与组氨酸残基的反应速度要慢得多,但这种活性是核糖核酸酶抑制的原因。

Features and Benefits

高品质化合物,适于多个研究领域

General description

2-碘乙酰胺是一种巯基-烷基化试剂,常用于生物化学、细胞培养和蛋白质组学研究中的肽图分析。作用方式与碘乙酸类似,主要通过共价结合蛋白质半胱氨酸残基上的巯基,防止二硫键形成。它在多种应用中发挥重要作用,比如在蛋白质二维电泳中,可作为去泛素化酶(DUB)抑制剂和肽酶抑制剂,减少拖尾并提高分辨率。也常用于肽质谱法从头测序中的样品制备。

它会与蛋白质中的半胱氨酸和组氨酸残基发生特定的相互作用,影响各种酶(如,核糖核酸酶)。与组氨酸缓慢反应的抑制特性令其适合蛋白酶研究(如,半胱氨酸蛋白酶)。碘乙酰胺的优势在于不可逆地抑制酶,形成稳定的蛋白衍生物,是蛋白质结构、蛋白行为和酶抑制研究的有用工具。

Legal Information

与蛋白质组系统合作开发的技术
Proteome Systems is a trademark of Proteome Systems Ltd

Other Notes

如需了解生化试剂系列的更多信息,请填写此表

Packaging

密封安瓿包装

Comparable product

產品號碼
描述
訂價

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Chronic 4 - Resp. Sens. 1 - Skin Sens. 1

存儲類別/等級

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges


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分析證明 (COA)

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Kristen L Koterba et al.
Journal of biotechnology, 157(1), 261-267 (2011-12-06)
During large-scale manufacturing of an IgG1 monoclonal antibody in Chinese hamster ovary (CHO) cells, reduction of the antibody's disulfide bonds was observed. We present evidence that mammalian thioredoxin 1 (TXN1) is the terminal enzyme responsible for this reduction event. We
Tanja Scherrer et al.
Genome biology, 12(1), R3-R3 (2011-01-15)
Glucose inhibition of gluconeogenic growth suppressor 2 protein (Gis2p) and zinc-finger protein 9 (ZNF9) are conserved yeast and human zinc-finger proteins. The function of yeast Gis2p is unknown, but human ZNF9 has been reported to bind nucleic acids, and mutations
Mirko Zaffagnini et al.
Antioxidants & redox signaling, 16(1), 17-32 (2011-06-29)
Cysteines (Cys) made acidic by the protein environment are generally sensitive to pro-oxidant molecules. Glutathionylation is a post-translational modification that can occur by spontaneous reaction of reduced glutathione (GSH) with oxidized Cys as sulfenic acids (-SOH). The reverse reaction (deglutathionylation)
Valentina R Minciacchi et al.
Oncotarget, 6(13), 11327-11341 (2015-04-11)
Large oncosomes (LO) are atypically large (1-10 µm diameter) cancer-derived extracellular vesicles (EVs), originating from the shedding of membrane blebs and associated with advanced disease. We report that 25% of the proteins, identified by a quantitative proteomics analysis, are differentially
Elena B Lugli et al.
Arthritis research & therapy, 17, 9-9 (2015-01-21)
Smoking is a well-established risk factor for rheumatoid arthritis (RA), and it has been proposed that smoking-induced citrullination renders autoantigens immunogenic. To investigate this mechanism, we examined human lung tissue from 40 subjects with defined smoking status, with or without

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Rapid trypsin digest kit yields reliable results in less than 2 hours for mass spectrometry analysis.

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.

條款

使用過濾器輔助樣品製備 (FASP) 優化 LC-MS/MS 工作流程,以進行單株抗體 adalimumab (Humira) 的低假象胰蛋白酶消化和勝肽圖譜製作。

An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).

Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. what's the solubility in pure ethanol? Do you offer smaller aliquots?

    1 answer
    1. This product is currently only offered in the 56 mg pack size. The solubility is tested in acetone at 50 mg/mL and it is also listed as being soluble in water at 50 mg/mL. According to a literature review, it should also be soluble in ethanol, but a concentration is not stated so experimentation will be necessary to determine the optimal solubility parameters.

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Reviews

Active Filters

  1. Cambridge
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Does exactly as needed

    Used to alkylate protein after reducing, prior to a trypsin digestion. Very easy to reconstitute, 1 vial covers many samples (I use at 15mM, reconstitute to 1.5M, the volume I use covers 200 samples). Works very quickly, 15 mins in the dark at room temp. Always shows as having worked in results.

    Helpful?

  2. 1 Ratings-only review

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