This product contains 3.7 g/L of NaHCO3.
D5796
DMEM - high glucose
With sodium Bicarbonate and ʟ-glutamine, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Dulbecco’s Modified Eagle’s Medium - high glucose
同義詞:
DME, DMEM
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產品名稱
杜氏改良 Eagle 培养基 - 高葡萄糖, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: yes
L-glutamine: yes
glucose: high
phenol red: yes
sodium pyruvate: no
HEPES: no
shipped in
ambient
storage temp.
2-8°C
Quality Level
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本產品 | D5671 | D6429 | D6546 |
|---|---|---|---|
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form liquid | form liquid | form liquid | form liquid |
| components NaHCO3: yes | components L-glutamine: no | components HEPES: no | components NaHCO3: yes |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
Application
Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。
General description
这种DMEM高葡萄糖培养基是一种不含添加因子（常见修改）如L-丙氨酰-L-谷氨酰胺、HEPES或丙酮酸钠的1x完全培养基。 它与原始DMEM高葡萄糖配方的不同点在于用吡哆醇代替了吡哆醛。 吡哆醛是一种不稳定的培养基成分。
Related product
產品號碼
描述
訂價
存儲類別/等級
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Instructions
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What is the NaHCO3 concentration? Conventional cell culture requires 5% of CO2. But it will depend on the NaHCO3 concentration. Does this product use 1.2 or 3.7 g/L?
1 answer-
Helpful?
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What are the product numbers for DMEM and EMEM used to grow the 293 and 293T cell lines?
1 answer-
To grow the 293 and 293T cells, the following product recommendations are provided:
For the 293 cell line (product number 85120602), ECACC recommends using EMEM media (EBSS) with 2mM Glutamine, 1% Non Essential Amino Acids (NEAA), and 10% FCS. ECACC uses the product number M4655 for this purpose.
For the 293T cell line (product number 12022001), ECACC recommends using DMEM media with 2mM Glutamine and 10% Fetal Bovine Serum (FBS). ECACC uses the product number D5796 media for sub-culturing this cell line.
Helpful?
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What is the conc. of L glutamine
1 answer-
The concentration of L-Glutamine in this product is 0.584 g/L. Please see the link below to review the DMEM Formulation Tables: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/340/109/d5796for.pdf
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pH는 몇으로 조정되어 있나요?
1 answer-
The pH specification of this product is 7.0-7.6. The exact value will be reported in the lot specific Certificate of Analysis. Please see the link below to review a sample Certificate of Analysis: https://www.sigmaaldrich.com/coa/SIGMA/D5796/RNBL9771.
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