Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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本產品 | WGA4 | WGA3 | WGA1 |
|---|---|---|---|
| technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
GenomePlex®全基因组扩增试剂盒采用基于基因组 DNA 随机片段化的专有技术，并将产生的小片段转换为两侧有通用引物结合位点的 PCR 可扩增文库分子。 WGA 采用通用寡核苷酸引物进行文库分子 PCR 扩增。它包括全基因组扩增所需的一切试剂，包括优化的酶 WGA DNA 聚合酶。它可用于多种应用，适用于来自各种来源的纯化基因组 DNA，包括血卡、全血、口腔拭子、土壤、植物和血清。它让研究人员可以生成具有代表性的、约 500 倍的基因组 DNA 扩增。
Features and Benefits
- 以最少的模板获得更高的产量: 在不到 3 小时内将纳克量的基因组 DNA 扩增为微克产量（约 10μ g）
- 纳克样品可在 –20 °C 下保存以备将来使用
- 可选择各种DNA来源：全血、口腔拭子、血卡、植物、土壤、&福尔马林固定石蜡包埋的组织（FFPE）
- 全基因组扩增 （WGA） DNA 聚合酶提高扩增准确度
- 全基因组无可检出的等位基因偏倚
- 兼容许多下游应用，例如 TaqMan ®检测、单核苷酸多态性 (SNP) 分析、比较基因组杂交 (CGH) 分析
Legal Information
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护： 5,789,224、5,618,711、6,127,155 和美国境外的权利要求，对应于过期的美国专利号 5,079,352。 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者′内部的研究用途。未通过明示、暗示或禁止反言形式授予购买者任何专利主张下的权利、执行任何专利方法的权利或开展任何形式的商业服务的权利，包括但不限于出于经济利益或其他商业考虑报告购买者′的活动结果。本品仅供研究使用。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系许可总监（Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA）获取。
GenomePlex is a registered trademark of Takara Bio USA, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
Other Notes
此试剂盒中提供的通用引物序列被认为是专有的。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存儲類別/等級
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Identification of key genes and pathways associated with different immune statuses of hepatitis B virus infection
Jin J, et al
Journal of Cellular and Molecular Medicine, 23, 7474-7489 (2019)
Novel cell enrichment technique for robust genetic analysis of archival classical Hodgkin lymphoma tissues
Juskevicius D, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 98, 1487-1499 (2018)
Zhong Zhao et al.
Nature, 465(7301), 1089-1092 (2010-06-26)
The classic phytohormones cytokinin and auxin play essential roles in the maintenance of stem-cell systems embedded in shoot and root meristems, and exhibit complex functional interactions. Here we show that the activity of both hormones directly converges on the promoters
Darius Juskevicius et al.
Laboratory investigation; a journal of technical methods and pathology, 98(11), 1487-1499 (2018-08-09)
Approximately 15% of patients with classical Hodgkin lymphoma (cHL) die after relapse or progressive disease. Comprehensive genetic characterization is required to better understand its molecular pathology and improve management. However, genetic information on cHL is hard to obtain mainly due
Jinglan Jin et al.
Journal of cellular and molecular medicine, 23(11), 7474-7489 (2019-10-01)
We aimed to identify key genes and pathways associated with different immune statuses of hepatitis B virus (HBV) infection. The gene expression and DNA methylation profiles were analysed in different immune statuses of HBV infection. Differentially expressed genes (DEGs) and
文章
aCGH 技術可增強染色體分析，每個樣本需要大量 DNA 輸入。
條款
Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, This protocol provides a simple and convenient method to extract genomic DNA from a blood card. Once the DNA has been extracted, it can then be amplified using the amplification protocol
Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.
Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.
GenomePlex® Whole Genome Amplification 可有效地從動物樣本中萃取 DNA 進行基因組分析。
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What is the Department of Transportation shipping information for this product?
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Is the WGA2 GenomePlex® Amplification Kit polymerase compatible with TA cloning?
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WGA polymerase is compatible with TA cloning with the following alteration to the PCR step: Be sure to include a 7 to 30 minute extension at 72°C after the last cycle to ensure that all PCR products are full length and 3´ adenylated.
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Can I amplify single stranded DNA with WGA2, GenomePlex® Amplification Kit?
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When starting with single stranded starting materials, we recommend (1) skipping the fragmentation heat step, although the buffer should be added and (2) increasing the PCR cycles from 14 to 20. Note that if the ssDNA is actually cDNA from polyadenylated RNA, the kit will likely not give good representation of the input material, as the poly(T) ends constitute a large, non-random fraction.
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Can I use Product WGA2, GenomePlex® Amplification Kit, to amplify DNA from a single cell?
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Using this kit to amplify DNA from a single cell is not recommended. We recommend using the GenomePlex Single Cell WGA Kit (WGA4) for such application. WGA4 was developed for use with single cells and includes an optimized cell lysis protocol which has been incorporated into the fragmentation step.
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If starting with fragmented DNA, what is the smallest size fragment which can be successfully amplified with Product WGA2, GenomePlex® Amplification Kit, and do I still need to do the fragmentation step?
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The kit works best for fragments 400 bp and larger. When starting with fragmented DNA, we recommend: (1) skipping the fragmentation heat step, although the buffer should be added, and (2) increasing the PCR cycles from 14 to 20.
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What is the major difference between WGA1 and WGA2 kits?
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Functionally, WGA1 and WGA2 kits are identical. The only difference between the two kits is that WGA2 is supplied with the WGA polymerase.
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