Supelco® HPLC Method Transfer Calculator
sigma-aldrich
Supelco® HPLC Method Transfer Calculator
sigma-aldrich
Supelco® HPLC Method Transfer Calculator
The scaled gradient method is based on estimates of column volumes and is therefore only an approximation. More precise method scaling requires actual measured values of column volumes. Column volume is a function of particle size, particle structure, and column packing.
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