Achieve High Virus Titers in Your AAV Development
Cell Culture Media for Sf-9 and HEK293
Optimized Cell Culture Media for AAV Manufacturing: Designed to get you up and running ASAP
Whether you’re working with Sf-9 insect cells or human HEK/293 cells in your AAV production, look to our expertise in media to quickly maximize your virus titers. Extensively tested, our media allow you to achieve your productivity objectives.
- For insect cells:
We have developed a chemically defined medium optimized for insect cell culture (EX-CELL® CD Insect Cell Medium) to maximize growth and productivity, especially for Sf21, Sf9, Tni, C6/36, and S2 cells, including the rhabdovirus-free Sf-RVN® cell line. - For HEK/293 cells:
Cellvento® 4HEK Medium is a chemically defined, animal component-free medium for suspension culture of HEK293 cell lines. Optimized for AAV production, it supports high full capsid titers.
Experience the benefits of our optimized cell culture media for yourself—click here to request your free sample and test it on your cells.
Why Use Our Media for Your AAV Production?
- Optimized Performance: Extensively tested for cell growth for AAV production
- Chemically defined, animal -component–free: Enhanced media stability, reduced protein oxidation, minimized contamination and variability risks, and supported regulatory compliance and reproducibility
- Versatility and Convenience: Supports all stages of their respective cell cultures, including growth, transfection, and production—as both an amplification and production medium.
The Proof is in the Results
EX-CELL CD Insect Cell Medium
The EX-CELL® CD Insect Cell Medium is a chemically defined medium specifically optimized for the growth and productivity of insect cell lines, including our Sf9 rhabdovirus-negative Sf-RVN® Insect Cell Line. This medium has demonstrated significant improvements in cell growth and protein production compared to traditional serum-based formulations.
For detailed insights into its development and performance, read our technical article here.
Interested to learn how to further improve AAV production? Download our application note whitepaper on tailored feeding strategies with Cellvento® Modifeed Prime feed COMP to boost AAV titers.
Cellvento® 4HEK Medium
Cellvento® 4HEK Medium is an advanced chemically defined, animal-component-free medium designed for the optimized production of AAV in HEK293 cell lines. This medium supports high viable cell densities and AAV titers, ensuring efficient production processes.
Read our technical article to learn more about its formulation and the results achieved.
Related Resources
- Development of a Novel Sf9 Rhabdovirus-Negative Cell Line (Sf-RVN®) and Companion Chemically Defined Medium
See how the Sf9 rhabdovirus-free cell line was developed and how we’ve developed a companion chemically-defined insect cell media for protein and viral expression.
- Development of a Novel Cell Culture Medium for AAV Production With Multiple HEK293 Lineages and Process Optimization
Read our white paper on the development of a novel cell culture medium for AAV production with multiple HEK293 lineages and process optimization.
- Scalability Challenges of Viral Vector Manufacturing
Scaling up viral vector production using adherent cell culture systems is challenging. Learn how suspension cell culture systems benefit large-scale bioprocessing.
- Bioprocessing And Scale-Up Of Suspension HEK293 Cells For AAV Manufacturing
To address scalability challenges of AAV manufacturing, we developed an HEK293 suspension cell line that can be used across many serotypes. Get the data in this article.
- Bioreactor Process Development and Scale-Up for Transient Transfection-Based Lentivirus Manufacturing in Suspension Cell Culture
A step-by-step overview of suspension-based, transient transfection bioreactor process development and scaleup of lentivirus production.
- Cell and Gene Therapy Manufacturing: Upstream Processing for AAV and Lentivirus Platforms
Minimize the complexities of cell and gene therapy production. Switch to upstream viral vector platforms to enhance scalability and simplify GMP manufacturing.
- High Performing Platforms for Lentivirus and AAV Manufacturing
Upstream gene therapy optimization maximizes viral vector titers; partner with technology experts for HEK293, HEK293T, and Sf9 cell solutions.
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