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MilliporeSigma

Bioprocessing Cell Culture Media & Feeds

This image shows two scientists in the MLab with bioreactors in the background.

For decades, biopharmaceutical companies have relied on our high-performing cell culture media (CCM) to intensify their processes and optimize production. Whether you are improving your cell culture process, perfecting your vaccine production, advancing your cell and gene therapy process, or pursuing tailored solutions meeting your specific cell culture process needs, our CCM and feeds empower you to achieve robust and efficient results.

What makes our media unique?

  • Extensive Portfolio: Explore our comprehensive range of media and feeds, featuring both established and innovative formulations for process intensification of monoclonal antibodies and novel modalities such as cell and gene therapies.
  • Unrivaled Quality and Performance: Enhance cell culture productivity with our chemically defined, animal origin-free media and feeds. Designed to streamline upstream workflows across all applications, our rigorously tested products ensure improved safety, regulatory compliance, and consistent performance.
  • Tailored Solutions: Rely on our global manufacturing network for customized dry powder and liquid media, designed to meet your unique requirements at any scale and for any application, ensuring a perfect match.
  • Expert Technical Support: Get top-tier application support from a dedicated team with expertise in various cell types, biologics, metabolic needs, and process requirements. We are fully committed to meeting your business continuity and process needs.
  • Risk Mitigation Support: Leverage our Emprove® CCM documentation to gain full transparency and comprehensive information needed for your qualification, risk assessment, and process optimization efforts

Section Overview



CHO Media and Feeds for Therapeutic Protein Production and Next Generation Biopharmaceuticals

In CHO cell culture, process intensification, media consistency and easy handling are critical. Our chemically defined formulations for cell line development, seed train, fed-batch, and perfusion are meticulously designed to support cell vitality and maximize productivity of CHO-based applications. We leverage innovative solutions such as modified amino acids, a novel poloxamer, low-impurity iron, and compacted CCM to align with novel process designs and facilitate upstream process intensification. In fed-batch processes, our highly concentrated one-part pH-neutral feeds support a streamlined workflow, replenishing depleted nutrients and reducing feed volume, ultimately increasing product yield.

Explore our Cellvento® ModiFeed platform supporting your individual feeding strategy:

Insect Cell Media for Vaccine and Gene Therapy Production

The production of recombinant proteins, virus-like particles, and adeno-associated viruses (AAV) for vaccines and gene therapy with insect cells demands specialized media that deliver outstanding performance and quality. Our chemically defined media is tailored to support robust growth of multiple insect cell lines, such as Sf-RVN®, Sf-9, Sf-21, S2, Tni and C636.

Explore our key products for excellent productivity and high-quality titers in insect cell culture:

HEK293 Media for Production of Gene Therapy

The human cell line HEK293 is widely used in the production of viral vectors for gene therapy and gene-modified cell therapy. Our chemically defined, animal-component-free HEK293 media is specifically formulated for optimal cell expansion, transient transfection, and high titers in AAV and lentivirus production, supporting suspension cell culture and a broad range of transfection agents, including cationic polymers such as PEI.

Discover our key products for excellent growth and productivity of HEK cell lines:

Custom Cell Culture Solutions

Individual applications require dedicated solutions. Whether you require dry powder or liquid formulations, for pre-GMP or GMP applications, small- or large-scale production, our global network of technical experts and manufacturing sites is committed to providing you with a solution tailored to your needs.

Find more information on our custom offering here:


Related Product Resources







Related Webinars

A digital illustration of a laptop displaying a simplistic human figure next to a pink rectangle, all set against a yellow background.
Turnkey Solutions for Upstream Cell Culture Processes

In this webinar, you will learn common challenges faced in upstream development, benefits of a one-step & neutral pH feed optimization strategy, potential application of chemically defined hydrolysates, and benefits of supplement targeted at adjusting glycosylation attributes.

A digital illustration of a laptop displaying a simplistic human figure next to a pink rectangle, all set against a yellow background.
Media and Process Development for Seed Train Intensification

In this webinar, you will learn how combining media specifically designed for seed train, production and harvest intensification, can increase the cell specific productivity (Qp) in the final production stage.

A digital illustration of a laptop displaying a simplistic human figure next to a pink rectangle, all set against a yellow background.
A Cost Analysis and Evaluation of Perfused Seed Train Scenarios Through Process Modeling

In this webinar, you will learn different variations of perfused seed train, potential benefits from high seed fed batch vs. traditional fed batch, impact of perfused seed trains in single bioreactor vs. multiple bioreactor scenarios, and impact of perfused seed trains in multiple production/seed train ratio scenarios.

A digital illustration of a laptop displaying a simplistic human figure next to a pink rectangle, all set against a yellow background.
Monitoring and Quality Control of Cell Culture Media Preparation Using Raman Spectroscopy

Explore the possibilities of using Raman spectroscopy for quality control of cell culture media preparation.

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