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IPFL20200

Immobilon® -FL PVDF Membrane

10 sheets, 20cm x 20 cm, 0.45 µm pore size, transfer membrane with low background fluorescence

Immobilon®-FL PVDF Membrane

Synonym(s):

Western blotting membrane, blotting membrane, transfer membrane

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10 EA

$455.00

$455.00

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane
plain filter
white filter
Detection method:
chemiluminescent
colorimetric
fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red

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Product Name

Immobilon®-FL PVDF Membrane, 10 sheets, 20 cm x 20 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

material

PVDF membrane
plain filter
white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

20 cm × 20 cm

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin)
 205 μg/cm2 adsorption capacity (BSA)
 300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red

detection method

chemiluminescent
colorimetric
fluorometric

shipped in

ambient

Quality Level

400

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Immobilon®-FL PVDF Membrane 10 sheets, 20 cm x 20 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Millipore

IPFL20200

Immobilon®-FL PVDF Membrane

Immobilon®-FL PVDF Membrane 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Millipore

IPFL00010

Immobilon®-FL PVDF Membrane

Immobilon®-FL PVDF Membrane 10 sheets, 10 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Millipore

IPFL10100

Immobilon®-FL PVDF Membrane

Millipore

Millipore

IPFL00005

Immobilon®-FL PVDF Membrane

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

material

PVDF membrane, white filter, plain filter

material

PVDF membrane, plain filter, white filter

material

PVDF membrane, plain filter, white filter

material

PVDF membrane, plain filter, white filter

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

detection method

chemiluminescent, fluorometric, colorimetric

detection method

chemiluminescent, colorimetric, fluorometric

detection method

chemiluminescent, colorimetric, fluorometric

detection method

chemiluminescent, colorimetric, fluorometric

compatibility

for use with Amido black, for use with Coomassie brilliant blue, for use with CPTS, for use with Ponceau-S red

compatibility

for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

compatibility

for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red

compatibility

for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

Application

Immobilon®-FL PVDF Membrane has been used in western blotting.[1][2]

Features and Benefits

Immobilon®-FL Membrane:
  • Designed for optimal fluorescent immunodetection
  • Extremely low background improves the sensitivity of all fluorescence detection protocols
  • suitable for all commonly used fluorescent probes at all excitation and emission wavelengths
  • compatible with standard blocking agents and buffers
  • Ideal for multiplexing and chemifluorescence detection

General description

The Immobilon®-FL transfer membrane has a nominal pore size of 0.45 μm and is made up of polyvinylidene fluoride (PVDF) microporous membrane for binding proteins transferred from a variety of gel matrices. This membrane is hydrophobic and offers a uniformly controlled pore structure with a high binding capacity for biiomolecules. The membrane exhibits very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection. In addition, the Immobilon®-FL membrane can be used for standard chemiluminescent or chromogenic detection. The Immobilon®-FL is recommended for optimal blotting of proteins with molecular weights greater than 20,000.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J L Portis et al.
Journal of virology, 83(12), 6079-6086 (2009-04-03)
A small group of ecotropic murine retroviruses cause a spongiform neurodegenerative disease manifested by tremor, paralysis, and wasting. The neurovirulence of these viruses has long been known to be determined by the sequence of the viral envelope protein, although the
Shaoqiu Jiang et al.
Cell cycle (Georgetown, Tex.), 18(11), 1241-1253 (2019-05-14)
Researches have been focusing on the role of Slit2 in angiogenesis, specifically in cell migration and vessel permeability. Nevertheless, the role of Slit2-N, the bioactive fragment of Slit2, in the proliferation of vascular endothelia in choroidal neovascularization and some related
Camilla Nehammer et al.
eLife, 8 (2019-12-05)
Appropriate regulation of autophagy is crucial for clearing toxic proteins from cells. Defective autophagy results in accumulation of toxic protein aggregates that detrimentally affect cellular function and organismal survival. Here, we report that the microRNA miR-1 regulates the autophagy pathway

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