PMSF-RO

Roche

PMSF

Phenylmethylsulfonyl fluoride

Synonym(s):
Phenylmethanesulfonyl fluoride, α-Toluenesulfonyl fluoride, phenylmethylsulfonyl fluoride, Benzylsulfonyl fluoride, Phenylmethylsulfonyl fluoride, PMSF
Empirical Formula (Hill Notation):
C7H7FO2S
CAS Number:
Molecular Weight:
174.19
Beilstein/REAXYS Number:
2088311
MDL number:
PubChem Substance ID:

Quality Level

assay

98% (TLC)

mol wt

174.2

packaging

pkg of 10 g (10837091001)
pkg of 25 g (11359061001)

manufacturer/tradename

Roche

concentration

17-170 μg/mL

application(s)

western blot: suitable

pKa 

7.0

mp

92-94 °C

solubility

>10 mg/mL (Soluble to >10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at +25°C.)

shipped in

ambient

storage temp.

20-25°C

SMILES string

FS(=O)(=O)Cc1ccccc1

InChI

1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2

InChI key

YBYRMVIVWMBXKQ-UHFFFAOYSA-N

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Specificity

Inhibits serine proteases such as chymotrypsin, trypsin, and thrombin, and the cysteine protease papain (reversible by DTT treatment). Does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.

Application

PMSF (C7H7O2SF) inhibits serine proteases such as chymotrypsin, trypsin, thrombin, and the cysteine protease papain (reversible by DTT treatment). It does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.
PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting.

Biochem/physiol Actions

PMSF (phenylmethylsulfonyl fluoride) functions as a non-specific inhibitor of multiple proteases and other enzymes, such as palmityl coenzyme A deacylase, arylsulfatase A, acetylcholinesterase, trypsin and chymotrypsin. It is an irreversible inhibitor, and sulfonylates hydroxyl groups of the serine residues present in the active site of enzymes.

Quality

Purity: Chromatographically homogeneous

Preparation Note

Activator: PK requires Mg2+ (or Mn2+, CO2+) and K+ (or NH4+, Rb+) for full activity.
Working concentration: 17 to 170 μg/ml (0.1 to 1 mM)
Working solution: Due to the short half-life of PMSF, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may have a half-life of only 35 minutes. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
Storage conditions (working solution): 15 to 25 °C
Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C.

Reconstitution

Soluble to > 10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at 15 to 25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.
Due to a short half-life, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may only have a half-life of 35 min. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.

RIDADR

UN 2923 6.1(8) / PGII

Certificate of Analysis
Certificate of Origin
D R Compton et al.
The Journal of pharmacology and experimental therapeutics, 283(3), 1138-1143 (1998-02-12)
Anandamide is an putative endogenous cannabinoid ligand that produces pharmacological effects similar to those of Delta9-tetrahydrocannabinol, the principle psychoactive constituent in marijuana. There is considerable evidence that the enzyme inhibitor phenylmethylsulfonyl fluoride (PMSF) is capable of altering the actions of...
Hongjuan Yu et al.
PloS one, 8(11), e80815-e80815 (2013-11-16)
A kinase interacting protein 1 (AKIP1) is a molecular regulator of protein kinase A and nuclear factor kappa B signalling. Recent evidence suggests AKIP1 is increased in response to cardiac stress, modulates acute ischemic stress response, and is localized to...
Guilian Tian et al.
The Journal of biological chemistry, 284(28), 18980-18993 (2009-05-09)
Clarin-1 is the protein product encoded by the gene mutated in Usher syndrome III. Although the molecular function of clarin-1 is unknown, its primary structure predicts four transmembrane domains similar to a large family of membrane proteins that include tetraspanins....
Ahmed M Kotb et al.
Breast cancer research : BCR, 13(5), R104-R104 (2011-10-28)
E-cadherin (E-cad; cadherin 1) and N-cadherin (N-cad; cadherin 2) are the most prominent members of the cadherin family of cell adhesion molecules. Although they share many structural and functional features, they are expressed in an almost mutually exclusive manner in...

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