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RTRYP-RO

Roche

Trypsin recombinant, Proteomics Grade

recombinant enzyme expressed in Pichia pastoris

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Synonym(s):
Trypsin
Enzyme Commission number:
3.4.21.4 (BRENDA, IUBMB)

recombinant

expressed in Pichia pastoris

Quality Level

100

Assay

95% (gel filtration)

form

lyophilized

specific activity

≥150 units/mg protein

packaging

pkg of 4 × 100 μg (03708969001)
pkg of 4 × 25 μg (03708985001)

manufacturer/tradename

Roche

storage temp.

−20°C

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T7575TRYPSEQM-ROMAK290
Trypsin recombinant, Proteomics Grade recombinant enzyme expressed in Pichia pastoris

Roche

RTRYP-RO

Trypsin recombinant, Proteomics Grade

Sigma-Aldrich

Sigma-Aldrich

T7575

Trypsin Singles, Proteomics Grade

Trypsin Sequencing Grade, modified from bovine pancreas

Roche

TRYPSEQM-RO

Trypsin Sequencing Grade, modified

Trypsin Activity Colorimetric Assay Kit sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK290

Trypsin Activity Colorimetric Assay Kit

recombinant

expressed in Pichia pastoris

recombinant

-

recombinant

-

recombinant

-

assay

95% (gel filtration)

assay

-

assay

-

assay

-

form

lyophilized

form

ready-to-use solution

form

lyophilized (salt-free)

form

-

specific activity

≥150 units/mg protein

specific activity

≥10,000 BAEE units/mg protein

specific activity

-

specific activity

-

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

Roche

manufacturer/tradename

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

General description

This trypsin preparation is generated from the recombinant Pichia pastoris strain and is, thus, free of chymotrypsin. Trypsin recombinant, Proteomics Grade, exhibits high specific activity.

Specificity

Serine endopeptidase, hydrolyzing specifically proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.

Application

Trypsin recombinant, Proteomics Grade, is used to specifically digest proteins separated by 2D gel electrophoresis or liquid chromatographic methods during sample preparation for mass spectrometry.

Preparation Note

Stabilizers: Ca2+ in mM concentration range
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin, α1-antitrypsin, APMSF, and antipain.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable for 1 month at -15 to -25°C. Avoid repeated freezing/thawing!

Reconstitution

Following reconstitution in 10 mM HCl, Trypsin, recombinant, proteomics grade is stable at -15 to -25 °C for up to one month. After first reconstitution the product must be stored in appropriate aliquots to avoid repeated freezing and thawing.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H334 - H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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