TRYPSEQM-RO
Roche
Trypsin Sequencing Grade, modified
from bovine pancreas
Synonym(s):
Trypsin
About This Item
Recommended Products
biological source
bovine pancreas
Quality Level
grade
protein sequencing grade
form
lyophilized (salt-free)
mol wt
24.000 g/mol
packaging
pkg of 4 × 100 μg (11418033001)
pkg of 4 × 25 μg (11418025001)
manufacturer/tradename
Roche
storage condition
(Keep container tightly closed in a dry and well-ventilated place.)
concentration
0.01-0.2 % (w/w)
technique(s)
protein sequencing: suitable
impurities
Chymotrypsin
color
white
optimum pH
8.0
solubility
10 g/L
suitability
suitable for protein modification
UniProt accession no.
application(s)
life science and biopharma
foreign activity
Contaminating activities corresponds
Chymotrypsin , contains
storage temp.
2-8°C
Gene Information
cow ... PRSS1(780933)
Related Categories
General description
Trypsin is a highly efficient and specific protease widely used in proteomics for protein digestion. It produces short peptides with specific characteristics that are compatible with current separation and identification methods such as liquid chromatography, mass spectrometry (MS).
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin,α1-antitrypsin, APMSF, and antipain.
Specificity
Application
It is used for:
- Protein-structure elucidation
- Tryptic mapping
- Fingerprinting analysis
- Sequence analysis
- Translocation studies
- Protein identification
- Protein digestion during lipoprotein preparation for liquid chromatography-tandem mass spectrometry (LC-MS/MS)
Quality
Preparation Note
Storage conditions (working solution): -15 to -25 °C
Trypsin Sequencing Grade, modified, is more resistant to autolysis, even at pH values in the neutral and weakly basic range. The enzyme can be used in high concentrations.
A solution in 1% acetic acid or 1 mM HCI can be used for up to one week when stored at 2 to 8° C. Stored in aliquots at -15 to -25 °C, the solution is stable for at least one year without loss of activity.
Storage and Stability
Other Notes
signalword
Danger
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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