PPB005

Phosphate Buffered Saline with 0.05% TWEEN^® 20, pH 7.4

pHast Pack^™, powder

• Synonym(s): PBS + 0.05% TWEEN^® 20, PBST
• NACRES: NA.21

Properties

• product line: pHast Pack^™
• form: powder

Description

General description

Phosphate Buffered Saline with 0.05% TWEEN^® 20 or PBST contains PBS buffer with a low-concentration detergent solution. It is a common wash buffer and due to the presence of detergent, it can easily remove excess components thus reducing non-specific binding on the membrane and microtiter plate wells.

Application

Suitable for use as a wash buffer in:

• Western blot
• ELISA
• Dot blot
• Immunohistochemistry

Features and Benefits

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Save time and minimize effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, 138 mM NaCl, 2.7 mM KCl; 0.05% Tween 20 (w/v), pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Legal Information

TWEEN is a registered trademark of Croda International PLC

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable