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PPB005

Sigma-Aldrich

Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4

pHast Pack, powder

Synonym(s):
PBS + 0.05% TWEEN® 20, PBST
NACRES:
NA.21

product line

pHast Pack

form

powder

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PPB002D1283D5652
product line

pHast Pack

product line

pHast Pack

product line

-

product line

-

form

powder

form

powder

form

liquid

form

powder

General description

Phosphate Buffered Saline with 0.05% TWEEN® 20 or PBST contains PBS buffer with a low-concentration detergent solution. It is a common wash buffer and due to the presence of detergent, it can easily remove excess components thus reducing non-specific binding on the membrane and microtiter plate wells.

Application

Suitable for use as a wash buffer in:

  • Western blot
  • ELISA
  • Dot blot
  • Immunohistochemistry

Features and Benefits

  • Ready-to-use buffer
  • No measuring and pH adjusting needed
  • Save time and minimize effort
  • Biological tests: free of DNase, RNase, protease, and nickase
  • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 10 mM Na2HPO4, 1.8 mM KH2PO4, 138 mM NaCl, 2.7 mM KCl; 0.05% Tween 20 (w/v), pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Legal Information

TWEEN is a registered trademark of Croda International PLC
pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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