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Sigma-Aldrich

Hydroxystilbamidine bis(methanesulfonate)

suitable for fluorescence, ≥96.0% (HPLC)

Synonym(s):
2-Hydroxystilbene-4,4′-dicarboxamidine bis(methanesulfonate), Fluoro-Gold
Empirical Formula (Hill Notation):
C18H24N4O7S2
CAS Number:
Molecular Weight:
472.54
MDL number:
PubChem Substance ID:
NACRES:
NA.32

Assay

≥96.0% (HPLC)

form

powder

fluorescence

λex 318 nm; λem 560 nm in TBE Buffer

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

CS(O)(=O)=O.CS(O)(=O)=O.NC(=N)c1ccc(cc1)\C=C\c2ccc(cc2O)C(N)=N

InChI

1S/C16H16N4O.2CH4O3S/c17-15(18)12-5-2-10(3-6-12)1-4-11-7-8-13(16(19)20)9-14(11)21;2*1-5(2,3)4/h1-9,21H,(H3,17,18)(H3,19,20);2*1H3,(H,2,3,4)/b4-1+;;

InChI key

YGNSQKCULHSJDC-HFPMQDOPSA-N

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assay

≥96.0% (HPLC)

assay

-

assay

≥90% (HPCE)

assay

≥99.0% (HPLC)

form

powder

form

powder

form

solid

form

powder or crystals

fluorescence

λex 318 nm; λem 560 nm in TBE Buffer

fluorescence

λex 560 nm; λem 580 nm in chloroform, λex 560 nm; λem ~580 nm in chloroform

fluorescence

λex 540 nm; λem 595 nm in DMSO

fluorescence

λex 365 nm; λem 445 nm in 0.1 M phosphate pH 6.5

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Hydroxystilbamidine bis (methanesulfonate), also known as Fluoro-Gold, is a retrograde fluorescent tracer. Chemically, Hydroxystilbamidine is a weak base and can cross cell membranes in its uncharged form.

A few properties of Hydroxystilbamidine bis (methanesulfonate) are:

  • Intense Fluorescence.
  • Extensive filling of dendrites.
  • High Resistance to fading.
  • No uptake by undamaged fibers.
  • No diffusion from labelled cells.
  • Highly compatible with other neuro-histochemical techniques.

Application

Hydroxystilbamidine bis(methanesulfonate), a fluorescent retrograde tracer (axonal transport), is used for neural mapping in microanatomical research.
Hydroxystilbamidine bis (methanesulfonate) or Fluoro-Gold (FG) is used to label neurons and investigate the early effects of hearing loss. It is used to study morphological features of extensive axonal projections within the CNS by labelling the cells.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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