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90C2

Sigma-Aldrich

Naphthol AS-D Chloroacetate (Specific Esterase) Kit

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Synonym(s):
Leder Stain
NACRES:
NA.47

description

Select reagents packaged in gelatin capsules

Quality Level

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

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This Item
91CHT100AG1041
shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

200

description

Select reagents packaged in gelatin capsules

description

Kit formulated with all liquid reagents

description

-

description

-

shelf life

Expiry date on the label.

shelf life

Expiry date on the label.

shelf life

Expiry date on the label

shelf life

-

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use

IVD

-

Application

Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. With the 90C2 kit, Naphthol AS-D Chloroacetate is enzymatically hydrolyzed by "specific esterase, " liberating a free naphthol compound. This then couples with a diazonium salt, forming highly colored deposits at sites of enzyme activity. This enzyme is usually considered specific for cells of granulocytic lineage. May be used to detect neutrophils in peripheral blood, bone marrow or tissue sections which have been paraffin embedded.

Kit Components Only

Product No.
Description

  • Dimethyl formamide (9010) 25 mL

  • Fast Corinth V Salt (9015) 10 capsules

Kit Components Also Available Separately

Product No.
Description
SDS

  • 2852Acid Hematoxylin Solution 100 mLSDS

  • 905Naphthol AS-D Chloroacetate 10 x 20SDS

  • 903CTRIZMAL™ 6.3 Buffer Concentrate, pH 6.3(concentrate), 0.2 M 50 mLSDS

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Chengjie Ma et al.
Molecular medicine reports, 22(1), 483-493 (2020-04-23)
Inactivation of the Hippo pathway protects the myocardium from cardiac ischemic injury. MicroRNAs (miRs) have been reported to play pivotal roles in the progression of myocardial infarction (MI). The present study examined whether miR‑93 could promote angiogenesis and attenuate remodeling
Feng Dong et al.
Circulation research, 106(3), 601-610 (2009-12-26)
Hypoxia inducible factor (HIF)-1alpha is a transcription factor stabilized by hypoxia. It regulates cytokines involved in the inflammatory response after ischemia and affects white blood cell (WBCs) function. The effect of HIF-1alpha on WBC function and inflammation following myocardial infarction
J Heijmans et al.
Oncogene, 32(9), 1202-1206 (2012-04-04)
Development of colon cancer is a multistep process that is regulated by intrinsic and extrinsic cellular signals. Extrinsic factors include molecular patterns that are derived from either pathogens (PAMPs) or cellular damage (DAMPs). These molecules can promote tumourigenesis by activation
John Zagorski et al.
Journal of immunology (Baltimore, Md. : 1950), 179(11), 7820-7826 (2007-11-21)
Right ventricular (RV) dysfunction is a strong risk factor for poor clinical outcome following pulmonary embolism (PE), the third most prevalent cardiovascular disease. Previous studies in our laboratory demonstrated that RV failure during PE is mediated, in part, by neutrophil-dependant

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