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APF
Alkaline Phosphatase Detection Kit, Fluorescence
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This Item | AP0100 | SAB5700875 | A7827 |
|---|---|---|---|
| Gene Information human ... ALPL(249) | Gene Information - | Gene Information human ... ALPL(249) | Gene Information - |
| technique(s) microbe id | specific enzyme detection: suitable | technique(s) - | technique(s) immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000 | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil |
| usage kit sufficient for 300 reactions (200 μl reactions) | usage sufficient for 100 tests | usage - | usage - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in wet ice |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
General description
The Alkaline Phosphatase (AP) Kit uses a fluorometric assay to detect AP activity. The kit capability is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis.
The gene for alkaline phosphatase (AP) commonly serves as a reporter gene and is used to determine the strength of promoters and enhancers, define the role of transcription factors, assess transfection efficiency and measure the success of molecular cloning attempts. The alkaline phosphatase (AP) kit uses a fluorometric assay to detect AP activity. The kit capability is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis.
Application
Alkaline Phosphatase Detection Kit, Fluorescence has been used to determine alkaline phosphatase (ALP) activity.[1]
Suitable for measuring the alkaline phosphatase (AP) or secreted alkaline phosphatase (SEAP)reporter gene activity. The Alkaline Phosphatase Kit can be used to determine the strength of promoters and enhancers, define the role of transcription factors, assess transfection efficiency, and measure the success of molecular cloning attempts.
Also suitable for detection of AP activity in pluripotent stem cells, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).
Also suitable for detection of AP activity in pluripotent stem cells, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).
Biochem/physiol Actions
Alkaline phosphatase (ALP) helps in hydrolyses and transphosphorylation of several phosphoric acid monoesters. It modulates various intracellular processes, that are associated with cell cycle, apoptosis, growth and signal transduction pathways.[2]
Features and Benefits
- Up to 100x more sensitive than colorimetric assays
- SEAP can be measured without sample destrutcion
- Rapid and reproducible results
- Safe and easy-to-use
Components
The Alkaline Phosphatase Kit contains:
50mL Fluorescent Assay Buffer ( B6558)
6 mL Dilution Buffer (B6433)
50 μl Alkaline Phosphatase Control Enzyme, 0.1 mg/mL (C9361)
2 x 1 mg 4-Methylumbelliferyl phosphate disodium (M8168)
50mL Fluorescent Assay Buffer ( B6558)
6 mL Dilution Buffer (B6433)
50 μl Alkaline Phosphatase Control Enzyme, 0.1 mg/mL (C9361)
2 x 1 mg 4-Methylumbelliferyl phosphate disodium (M8168)
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the Department of Transportation shipping information for this product?
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Is Product APF, Alkaline Phosphatase Detection Kit, Fluorescence, used to measure the alkaline phosphatase (AP) activity of cell lysates or the AP activity secreted by cells?
1 answer-
The technical bulletin provides procedures for both. The kit was originally designed for secreted alkaline phosphatase (SEAP), because the human placental SEAP standard that comes with the kit is a secreted enzyme. Its activity in the cell sample can be measured nondestructively and repeatedly over time using an aliquot of the culture medium. It saves time required for cell extract preparations.
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Is Product APF, Alkaline Phosphatase Detection Kit, used for fluorometric or colorimetric assay?
1 answer-
The assay used is fluorometric, which is more sensitive than the colorimetric assay.
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