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B6032

Sigma-Aldrich

BIS-TRIS hydrochloride

≥99.0% (titration)

Synonym(s):

2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane

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About This Item

Empirical Formula (Hill Notation):
C8H19NO5 · HCl
CAS Number:
Molecular Weight:
245.70
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

Quality Level

assay

≥99.0% (titration)

form

crystalline powder

useful pH range

5.8-7.2

pKa 

6.5

solubility

water: 0.5 g/mL, clear, colorless to very faintly yellow

application(s)

diagnostic assay manufacturing

storage temp.

room temp

SMILES string

Cl.OCCN(CCO)C(CO)(CO)CO

InChI

1S/C8H19NO5.ClH/c10-3-1-9(2-4-11)8(5-12,6-13)7-14;/h10-14H,1-7H2;1H

InChI key

VEYRVLHAMHQVTC-UHFFFAOYSA-N

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Related Categories

Application


  • Phos-tag SDS-PAGE systems for phosphorylation profiling of proteins with a wide range of molecular masses under neutral pH conditions.: This research introduces the use of BIS-TRIS hydrochloride in Phos-tag SDS-PAGE systems to profile protein phosphorylation. The system operates under neutral pH conditions, making it suitable for analyzing proteins with diverse molecular weights. This method enhances the resolution of phosphorylated proteins, facilitating detailed phosphorylation studies crucial for understanding cellular signaling and protein function (Kinoshita et al., 2012).

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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