C8482

Sigma-Aldrich

Protein Kinase A catalytic subunit human

>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

Synonym(s):
Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human
MDL number:
NACRES:
NA.32

Quality Level

recombinant

expressed in E. coli

assay

>90% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

>1000 units/mg protein

mol wt

43.5 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKACA(5566)

Biochem/physiol Actions

Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.

Physical form

Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.

Analysis Note

One unit will transfer 1 nanomole of phosphate from 32P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay).
Km (ATP) = 25 μM at 30 °C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Toshio Kitazawa et al.
The Journal of physiology, 587(Pt 14), 3587-3603 (2009-05-28)
Nitric oxide (NO) from endothelium is a major mediator of vasodilatation through cGMP/PKG signals that lead to a decrease in Ca(2+) concentration. In addition, NO-mediated signals trigger an increase in myosin light chain phosphatase (MLCP) activity. To evaluate the mechanism...
Chung-Lin Chou et al.
American journal of physiology. Cell physiology, 314(1), C99-C117 (2017-10-20)
The urea channel UT-A1 and the water channel aquaporin-2 (AQP2) mediate vasopressin-regulated transport in the renal inner medullary collecting duct (IMCD). To identify the proteins that interact with UT-A1 and AQP2 in native rat IMCD cells, we carried out chemical...
Tadahiko Matsumoto et al.
Scientific reports, 9(1), 8307-8307 (2019-06-06)
APOBEC3B cytidine deaminase (A3B) catalyzes cytosine into uracil in single-strand DNA and induces C-to-T mutations in genomic DNA of various types of tumors. Accumulation of APOBEC signature mutations is correlated with a worse prognosis for patients with breast cancer or...

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