C8482

Protein Kinase A catalytic subunit human

>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

• Synonym(s): Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human
• MDL number: MFCD03458215
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• assay: >90% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: >1000 units/mg protein
• mol wt: 43.5 kDa
• UniProt accession no.: P17612
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PRKACA(5566)

Description

Packaging

10 μg

Biochem/physiol Actions

Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.

Physical form

Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.

Analysis Note

One unit will transfer 1 nanomole of phosphate from ³²P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay).
K_m (ATP) = 25 μM at 30 °C, pH 7.4. K_m (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable