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C8482

Sigma-Aldrich

Protein Kinase A catalytic subunit human

>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

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Synonym(s):
Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human
MDL number:
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

assay

>90% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

>1000 units/mg protein

mol wt

43.5 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKACA(5566)

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Protein Kinase Cδ isozyme human

Sigma-Aldrich

P3738

Protein Kinase G Iβ human

Gene Information

human ... PRKACA(5566)

Gene Information

human ... PRKACB(5567)

Gene Information

human ... PRKCD(5580)

Gene Information

human ... PRKG1(5592)

specific activity

>1000 units/mg protein

specific activity

≥10 nmol/min·mg

specific activity

-

specific activity

≥1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

recombinant

expressed in E. coli

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected insect cells

recombinant

expressed in baculovirus infected Sf9 cells

mol wt

43.5 kDa

mol wt

protein apparent mol wt ~65 kDa (including N-terminal GST-tag of ~26kDa)

mol wt

74-79 kDa by SDS-PAGE

mol wt

76 kDa (monomer)

Biochem/physiol Actions

Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.

Physical form

Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.

Analysis Note

One unit will transfer 1 nanomole of phosphate from 32P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay).
Km (ATP) = 25 μM at 30 °C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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