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  • CS0780
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CS0780

Sigma-Aldrich

β-N-Acetylglucosaminidase Assay Kit

sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)

EC Number:
NACRES:
NA.84

usage

sufficient for 50 reactions (1 mL)
sufficient for 500 reactions (100 μL)

Quality Level

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... NAGLU(4669)

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usage

sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)

usage

sufficient for 100 colorimetric tests

usage

sufficient for 100 colorimetric tests

usage

-

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

100

Gene Information

human ... NAGLU(4669)

Gene Information

human ... GBA(2629), GBA2(57704), GBA3(57733)
mouse ... GBA(14466), GBA2(230101)
rat ... GBA(684536), GBA2(298399), GBA3(289687)

Gene Information

human ... GAA(2548)
mouse ... GAA(14387)
rat ... GAA(367562)

Gene Information

-

Application

The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.

Biochem/physiol Actions

N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.

Analysis Note

The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.

Kit Components Only

Product No.
Description

  • Dilution Buffer 8 mL

  • 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg

  • Citrate Buffer Solution, 0.09 M 100 mL

  • p-Nitrophenol Standard Solution, 10 mM 1 mL

  • β-N-Acetylglucosaminidase from Jack beans 1 vial

  • Sodium carbonate 5 g

Pictograms

Exclamation markHealth hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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