MilliporeSigma
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D2001

Sigma-Aldrich

Deoxyribonucleic acid sodium salt from Escherichia coli strain B

Type VIII

Synonym(s):
DNA
CAS Number:
EC Number:
MDL number:
eCl@ss:
32160414
NACRES:
NA.51

Quality Level

type

Type VIII

form

fibers

impurities

<2% protein

storage temp.

2-8°C

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Application

Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used to measure the melting temperature (Tm) and in differential scanning calorimetry (DSC) studies. It has also been used as a template sequence in the polymerase chain reaction (PCR).
Escherichia coli DNA has many uses in research. Escherichia coli DNA (ecDNA) is used in studies of DNA binding agents that modulate DNA structure and function. E. coli DNA is used in physicochemical studies of DNA behavior in solution, and to evaluate species-specific DNA structures and binding domains. This DNA is particularly useful for genomic analysis, including PCR, library construction in bacteriophage lambda, and in heterologous pre-hybridization and hybridization protocols.

Packaging

5 mg in glass bottle
10, 25 mg in poly bottle

Preparation Note

Prepared by procedure of Marmur, J., J. Mol. Biol., 3, 208 (1961).

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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