D2178

Sigma-Aldrich

Monoclonal Anti-Death Associated Protein Kinase antibody produced in mouse

clone DAPK-55, ascites fluid

Synonym(s):
Anti-DAP Kinase
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

DAPK-55, monoclonal

mol wt

antigen 160 kDa

contains

15 mM sodium azide

species reactivity

human, monkey

application(s)

immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:250 using COS-7 cell extract

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... DAP(1611)

General description

The gene is widely expressed in many tissues of embryonic and adult origin and gives rise to a single 6.3 Kb mRNA transcript. The protein consists of 1423 amino acids (160 kDa) and belongs to a family of calmodulin-dependent protein kinases. It contains 8 ankyrin repeats and 2 potential P-loop motifs apart from the kinase domain and the calmodulin regulatory domain.
Monoclonal Anti-Death Associated Protein (DAP) Kinase (mouse IgG1 isotype) is derived from the DAPK-55 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Death-associated protein 1 (DAP1) gene is mapped to human chromosome 9q34.1. It belongs to the DAP family.

Immunogen

recombinant human DAP kinase expressed in bacteria.

Application

Monoclonal Anti-Death Associated Protein Kinase antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Death-associated protein 1 (DAP1) participates in the regulation of cell growth and death including that of cancer cells. DAP-kinase has an intrinsic kinase activity towards itself that is regulated by calmodulin and calcium.
DAP-kinase is a novel, structurally unique, human serine/threonine kinase identified as a positive mediator of interferon-γ-induced programmed cell death. It has an intrinsic kinase activity towards itself that is regulated by calmodulin and calcium.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

KLHL39 suppresses colon cancer metastasis by blocking KLHL20-mediated PML and DAPK ubiquitination
Chen HY, et al.
Oncogene, 34(40), 5141-5141 (2015)
B Inbal et al.
Nature, 390(6656), 180-184 (1997-11-21)
DAP kinase is a new type of calcium/calmodulin-dependent enzyme that phosphorylates serine/threonine residues on proteins. Its structure contains ankyrin repeats and the 'death' domain, and it is associated with the cell cytoskeleton. The gene encoding DAP kinase was initially isolated...
Death-associated protein kinase increases glycolytic rate through binding and activation of pyruvate kinase
Mor I, et al.
Oncogene, 31(6), 683-683 (2012)
KLHL39 suppresses colon cancer metastasis by blocking KLHL20-mediated PML and DAPK ubiquitination.
Chen, et al.
Oncogene, 34, 5141-5151 (2020)
The role of DAPK-BimEL pathway in neuronal death induced by oxygen-glucose deprivation
He C, et al.
Neuroscience, 258(6), 254-262 (2014)

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