Anti-DNP antibody produced in rabbit was used to tag derivatized protein carbonyl groups in plasma by ELISA. It was used for differential cell count of zona-free blastocysts in IVF embryos.
Biochem/physiol Actions
2,4-Dinitrophenyl is an oxidative phosphorylation inhibitor and hinders adenosine triphosphate (ATP) synthesis. Due to its low excretion rate, 2,4-dinitrophenyl is considered toxic to human. It increases electron transportation, respiration and overall metabolism.
Analysis Note
Antiserum is tested for specificity and titer by immunoelectrophoresis; treated to remove lipoproteins..
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