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D9656

Sigma-Aldrich

Anti-DNP antibody produced in rabbit

whole antiserum

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Synonym(s):
Anti Dnp, Anti Dnp Antibody, Anti-DNP - Anti-DNP antibody produced in rabbit, Anti-Dnp, Anti-Dnp Antibody, Dnp Antibody
MDL number:
MFCD00162263
NACRES:
NA.46

biological source

rabbit

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:8,000

shipped in

dry ice

storage temp.

−20°C

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Anti-DNP antibody produced in rabbit whole antiserum

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conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

whole antiserum

antibody form

whole antiserum

antibody form

whole antiserum

antibody form

whole antiserum

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

technique(s)

indirect ELISA: 1:8,000

technique(s)

dot blot: suitable using α-catenin peptide amino acids 890-901 conjugated to BSA, immunohistochemistry (frozen sections): 1:2,000 using bovine kidney sections, indirect immunofluorescence: 1:2,000 using cultured MDBK cells, microarray: suitable, western blot: 1:4,000 using cultured MDBK cells extract

technique(s)

indirect ELISA: 1:8,000, quantitative precipitin assay: 1.6-2.4 mg/mL

technique(s)

indirect immunofluorescence: 1:20 using cultured cells, indirect immunofluorescence: 1:20 using human skeletal muscle sections, western blot: 1:100 using animal or human skeletal muscle.

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Immunogen

A DNP-BSA conjugate

Application

Anti-DNP antibody produced in rabbit was used to tag derivatized protein carbonyl groups in plasma by ELISA. It was used for differential cell count of zona-free blastocysts in IVF embryos.

Biochem/physiol Actions

2,4-Dinitrophenyl is an oxidative phosphorylation inhibitor and hinders adenosine triphosphate (ATP) synthesis. Due to its low excretion rate, 2,4-dinitrophenyl is considered toxic to human. It increases electron transportation, respiration and overall metabolism.

Analysis Note

Antiserum is tested for specificity and titer by immunoelectrophoresis; treated to remove lipoproteins..

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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