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F3133

Sigma-Aldrich

Fibroblast Growth Factor from bovine pituitary

suitable for cell culture

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Synonym(s):
FGF
CAS Number:
MDL number:
NACRES:
NA.77

biological source

bovine pituitary glands

Quality Level

form

lyophilized powder

potency

1-100 ng/mL

mol wt

16.4 kDa

packaging

pkg of 10 and 5X10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white to off-white

storage temp.

−20°C

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This Item
F5392F3685F5542
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

potency

1-100 ng/mL

potency

0.03-3.0 ng/mL ED50/EC50

potency

<1 ng/mL Biological Activity EC50

potency

0.287 ng/mL

mol wt

16.4 kDa

mol wt

16.4 kDa

mol wt

16.0 kDa

mol wt

15.5 kDa

packaging

pkg of 10 and 5X10 μg

packaging

pkg of 1 μg

packaging

pkg of 4X25 μg, pkg of 25 μg

packaging

pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

General description

Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface. There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34kDa and they possess a conserved sequence of 120 amino acids.

Application

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor from bovine pituitary has been used in in vitro testicular cell culture. It has also been used as a component of myogenic culture medium.

Biochem/physiol Actions

Fibroblast growth factors (FGF) actively participate in cellular responses including embryogenesis and organogenesis. It specifically controls cell proliferation, differentiation and migration. FGF is known to regulate nervous system functions, tissue repair mechanism and wound healing. It also controls angiogenesis of tumor cells. Abnormal FGF mediated signaling induces tumor angiogenesis, by stimulating cancer cell proliferation and survival. FGFs carries out its function by mediating through four receptor tyrosine kinases : FGF-receptors FGFR1, FGFR2, FGFR3 and FGFR4.

Quality

Although the major mitogenic component of this product is FGF2, the activity per unit weight of protein is less than that of purified FGF2.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.1 mg/ml BSA.

Preparation Note

Purified glands by a modification of the method of Gospodarowicz.

Analysis Note

The biological activity is measured using a 3-day MTT assay.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Modulation of osteogenic, adipogenic and myogenic differentiation of mesenchymal stem cells by submicron grooved topography
Wang PY, et al.
Journal of Materials Science. Materials in Medicine, 23(12), 3015-3028 (2012)
Characterization and In Vitro Differentiation of Korean Ring-Necked Pheasant (Phasianus colchicus) Male Germ Cells
Jeong DK, et al.
Journal of Embryology transfer , 29(4), 351-359 (2014)
Cellular signaling by fibroblast growth factor receptors
Eswarakumar VP, et al.
Cytokine & Growth Factor Reviews, 16(2), 139-149 (2005)
Fibroblast growth factor signalling: from development to cancer
Turner N and Grose R
Nature Reviews. Cancer, 10(2), 116-116 (2010)
Identification, culture, and characterization of germline stem cell-like cells in chicken testes
Jung JG, et al.
Biology of Reproduction, 76(1), 173-182 (2007)

Articles

Fibroblast Growth Factor Family (FGF)

Fibroblast growth factors in cell culture and various growth factors for your research

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