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F3133

Sigma-Aldrich

Fibroblast Growth Factor from bovine pituitary

lyophilized, suitable for cell culture

Synonym(s):

FGF

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

product name

Fibroblast Growth Factor from bovine pituitary, suitable for cell culture

biological source

bovine pituitary glands

Quality Level

form

lyophilized powder

potency

1-100 ng/mL

mol wt

16.4 kDa

packaging

pkg of 10 and 5X10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white to off-white

storage temp.

−20°C

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General description

Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface. There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34kDa and they possess a conserved sequence of 120 amino acids.

Application

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor from bovine pituitary has been used in in vitro testicular cell culture. It has also been used as a component of myogenic culture medium.

Biochem/physiol Actions

Fibroblast growth factors (FGF) actively participate in cellular responses including embryogenesis and organogenesis. It specifically controls cell proliferation, differentiation and migration. FGF is known to regulate nervous system functions, tissue repair mechanism and wound healing. It also controls angiogenesis of tumor cells. Abnormal FGF mediated signaling induces tumor angiogenesis, by stimulating cancer cell proliferation and survival. FGFs carries out its function by mediating through four receptor tyrosine kinases : FGF-receptors FGFR1, FGFR2, FGFR3 and FGFR4.

Quality

Although the major mitogenic component of this product is FGF2, the activity per unit weight of protein is less than that of purified FGF2.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.1 mg/ml BSA.

Preparation Note

Purified glands by a modification of the method of Gospodarowicz.

Analysis Note

The biological activity is measured using a 3-day MTT assay.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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Modulation of osteogenic, adipogenic and myogenic differentiation of mesenchymal stem cells by submicron grooved topography
Wang PY, et al.
Journal of Materials Science. Materials in Medicine, 23(12), 3015-3028 (2012)
Characterization and In Vitro Differentiation of Korean Ring-Necked Pheasant (Phasianus colchicus) Male Germ Cells
Jeong DK, et al.
Journal of Embryology transfer , 29(4), 351-359 (2014)
Cellular signaling by fibroblast growth factor receptors
Eswarakumar VP, et al.
Cytokine & Growth Factor Reviews, 16(2), 139-149 (2005)
Rocky S Tuan et al.
The Journal of the American Academy of Orthopaedic Surgeons, 21(5), 303-311 (2013-05-03)
Cartilage damaged by trauma has a limited capacity to regenerate. Current methods of managing small chondral defects include palliative treatment with arthroscopic débridement and lavage, reparative treatment with marrow-stimulation techniques (eg, microfracture), and restorative treatment, including osteochondral grafting and autologous
Jin Gyoung Jung et al.
Biology of reproduction, 76(1), 173-182 (2006-10-13)
We recently succeeded in inducing germline transmission by transferring chicken testicular cells into heterologous testes. This study was designed subsequently to identify pluripotent cells in the testicular cells, which would induce the germline transmission. Testicular cells retrieved from juvenile (4-wk-old)

Articles

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