NONH for all modes of transport
Any DNA polymerase with a 3’-5’ exonuclease activity is NOT recommended. These include Tli, Pfu,and Pwo. Those DNA polymerases that can be used include Taq, Tfl, and Tth. The kit has been optimized for use with Product No. D9307.
Sigma Aldrich does not recommend diluting the sample and re-assay as this can result in false negatives (sample diluted below the detection limits of the assay). Sigma Aldrich recommends that PCR inhibitors be removed using a DNA extraction with commercially available kits. (Product Codes G1N10, G1N70, NA2000).
A cell lysate can contain PCR inhibitors. Sigma Aldrich recommends that PCR inhibitors be removed using a DNA extraction with commercially available kits. (Product Codes G1N10, G1N70, NA2000).
The assay kits have a similar range of species detected (see product insert for listing). For Product No. MP0025, the components are supplied as individual reagents. For Product No. MP0035, the components are supplied in reaction tubes, already mixed and ready for addition of sample and Taq. The internal control for Product No. MP0035 is at 481 bp rather than 191 bp as found in Product No. MP0025.
The mycoplasma assay kit can be run on supernatants from either adherent or suspension cells. The centrifugation step during sample preparation will remove any cellular debris to limit PCR inhibitors.
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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