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P1435

Phosphatase, Acid from sweet potato

ammonium sulfate suspension, ≥10.0 units/mg protein (modified Warburg-Christian)

Synonym(s):

(Orthophosphoric-monoester phosphohydrolase [acid optimum] )

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About This Item

CAS Number:
9001-77-8
EC Number:
3.1.3.2 (BRENDA, IUBMB)
EC Number:
232-630-9
MDL number:
MFCD00131847
UNSPSC Code:
12352204
NACRES:
NA.54

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form

ammonium sulfate suspension

Quality Level

300

specific activity

≥10.0 units/mg protein (modified Warburg-Christian)

secondary activity

≤9.9 units/mg protein ATPase

mol wt

110 kDa

storage temp.

2-8°C

SMILES string

O(CC(O)C)CC#C

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3

InChI key

GZCWLCBFPRFLKL-UHFFFAOYSA-N

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This Item
SRE0048P1146P3752
Phosphatase, Acid from sweet potato ammonium sulfate suspension, ≥10.0 units/mg protein (modified Warburg-Christian)

Sigma-Aldrich

P1435

Phosphatase, Acid from sweet potato

Sigma-Aldrich

Sigma-Aldrich

SRE0048

Phosphatase, Acid from potato

Phosphatase, Acid from potato lyophilized powder, ≥3.0 units/mg solid

Sigma-Aldrich

P1146

Phosphatase, Acid from potato

Phosphatase, Acid from potato lyophilized powder, ≥0.5 unit/mg solid

Sigma-Aldrich

P3752

Phosphatase, Acid from potato

specific activity

≥10.0 units/mg protein (modified Warburg-Christian)

specific activity

3.0-10.0 unit/mg solid

specific activity

≥3.0 units/mg solid

specific activity

≥0.5 unit/mg solid

form

ammonium sulfate suspension

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

110 kDa

mol wt

-

mol wt

69 kDa

mol wt

69 kDa

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

400

Quality Level

300

Quality Level

200

secondary activity

≤9.9 units/mg protein ATPase

secondary activity

-

secondary activity

-

secondary activity

-

General description

Acid phosphatase from sweet potato is a phosphomonoesterase, which can appear in multiple molecular forms of similar molecular mass but with different isoelectric points. [1]

Application

Potato acid phosphatase is used as a dephosphorylating reagent in thiochrome assays for the detection of thiamin in biological samples. In this assay, it was shown to be more effective than acid phosphatases from wheat germ or α-amylase. [2]
Sweet potato acid phosphatase has been used in a study as a pre-column enzyme reactor via a covalent bond with glutaraldehyde and aminopropyl controlled-pore glass. [3] It has also been used in a study to investigate the use of tyrosine, histidine, and cysteine as ligands for Mn(III) in sweet potato phosphatase. [4]

Biochem/physiol Actions

Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7 by the following reaction: APaseR-PO4 + H2O --------------- R-OH + HOPO3 2+Their function in the production, transport, and recycling of phosphate is critical for the metabolic and energy transduction processes of the cell. As a group, Apases may be as important as kinases in regulatory processes.

Physical form

Suspension in 1.8 M (NH4)2SO4, 10 mM MgCl2, pH 5.3

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLBS7825
096K7351V
096K7351
071K7025
092H7045

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An unstable manganese(III) complex incorporating ligand donor types proposed for an acid phosphatase from sweet potato: (p-nitrobenzenethiolato)[N,N'-ethylenebis(salicylideneaminato)]manganese(III)
Gohdes, J. and W. Armstrong
Inorganic Chemistry, 27, 1841-1842 (1988)
D T Wyatt et al.
Clinical chemistry, 35(11), 2173-2178 (1989-11-01)
We analyzed extensively a modified thiochrome method for thiamin analysis. Acid phosphatase (EC 3.1.3.2) from potato was superior to either alpha-amylase or acid phosphatase from wheat germ as a dephosphorylating agent. Timing of cyanogen bromide exposure was important, but the
Tatsuya Kusudo et al.
Bioscience, biotechnology, and biochemistry, 67(7), 1609-1611 (2003-08-13)
Purple acid phosphatase (PAP) was purified from sweet potato dry powder, which is used as a food additive. Spectrometric and enzymatic analyses, and analysis of the amino-terminal sequence indicated that the purified purple acid phosphatase was PAP1. High activity in
Mina Solhtalab et al.
Environmental science & technology, 56(22), 16441-16452 (2022-10-26)
Among ubiquitous phosphorus (P) reserves in environmental matrices are ribonucleic acid (RNA) and polyphosphate (polyP), which are, respectively, organic and inorganic P-containing biopolymers. Relevant to P recycling from these biopolymers, much remains unknown about the kinetics and mechanisms of different
G Schenk et al.
Archives of biochemistry and biophysics, 370(2), 183-189 (1999-10-08)
Purple acid phosphatases comprise a family of binuclear metal-containing acid hydrolases, representatives of which have been found in animals, plants, and fungi. The goal of this study was to characterize purple acid phosphatases from sweet potato tubers and soybean seeds
View All Related Papers

Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocols

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

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