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P8563

Sigma-Aldrich

Polyuridylic acid–Agarose

lyophilized powder, matrix polyacrylhydrazido-agarose

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Synonym(s):
Poly(U)–Agarose potassium salt
MDL number:
MFCD00165499
NACRES:
NA.56

form

lyophilized powder

Quality Level

200

extent of labeling

0.1-1.0 mg per mL

matrix

polyacrylhydrazido-agarose

matrix attachment

ribose moiety after periodate oxidation

swelling

1 g swells to ~4 mL

capacity

≥0.2 mg/mL binding capacity (poly(A) (P 9403))

storage temp.

−20°C

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A2767P8901P9827
Polyuridylic acid–Agarose lyophilized powder, matrix polyacrylhydrazido-agarose

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P8563

Polyuridylic acid–Agarose

Adenosine 5′-triphosphate–Agarose lyophilized powder

Sigma-Aldrich

A2767

Adenosine 5′-triphosphate–Agarose

Sigma-Aldrich

Sigma-Aldrich

P8901

Phenyl-Agarose

Polycytidylic acid–Agarose lyophilized powder

Sigma-Aldrich

P9827

Polycytidylic acid–Agarose

extent of labeling

0.1-1.0 mg per mL

extent of labeling

1-5 μmol per mL

extent of labeling

-

extent of labeling

0.25-1.0 mg per mL

matrix

polyacrylhydrazido-agarose

matrix

cross-linked 4% beaded agarose

matrix

Cross-linked 4% beaded agarose

matrix

cross-linked 4% beaded agarose

matrix attachment

ribose moiety after periodate oxidation

matrix attachment

C-8

matrix attachment

amino

matrix attachment

amino

swelling

1 g swells to ~4 mL

swelling

-

swelling

-

swelling

1 g swells to ~5 mL

capacity

≥0.2 mg/mL binding capacity (poly(A) (P 9403))

capacity

-

capacity

20-50 mg binding capacity (human serum albumin)(per ml of packed gel), ≥4 mg binding capacity (β-lactoglobulin dimer)(per ml of packed gel)

capacity

-

Application

Polyuridylic acid-agarose is used in protein chromatography, affinity chromatography and specialty resins. Polyuridylic acid has been used to study insulin receptors and to develop polyclonal antibodies against RNase L.

Physical form

Lyophilized powder stabilized with lactose

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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C F Wright et al.
The Journal of biological chemistry, 276(44), 40680-40686 (2001-09-08)
Vaccinia virus gene expression is temporally regulated, and three gene classes have been identified: early, intermediate, and late. Several virus-encoded proteins and an activity designated VLTF-X are required for maximum transcription in vitro of a template containing a late promoter.
Ling Du et al.
RNA (New York, N.Y.), 11(9), 1340-1347 (2005-07-27)
Activity-dependent changes in protein synthesis modify synaptic efficacy. One mechanism that regulates mRNA translation in the synapto-dendritic compartment is cytoplasmic polyadenylation, a process controlled by CPEB, the cytoplasmic polyadenylation element (CPE)-specific RNA binding protein. In neurons, very few mRNAs are
S H Kim et al.
The EMBO journal, 11(6), 2319-2326 (1992-06-01)
Unlike autocatalyzed self-splicing reactions, nuclear pre-mRNA splicing requires transacting macromolecules and ATP. A protein encoded by the PRP2 gene of Saccharomyces cerevisiae is required, in conjunction with ATP, for the first cleavage-ligation reaction of pre-mRNA splicing. In this study, we
E A Zemskov et al.
Journal of virology, 74(15), 6784-6789 (2000-07-11)
The Bombyx mori nucleopolyhedrovirus (BmNPV) genome contains five related members of the bro gene family, all of which are actively expressed in infected BmN cells. Although their functions are unknown, their amino acid sequences contain a motif found in all
Shijun Cheng et al.
The Journal of biological chemistry, 282(35), 25247-25258 (2007-07-04)
The poly(A)-binding protein (PABP), a protein that contains four conserved RNA recognition motifs (RRM1-4) and a C-terminal domain, is expressed throughout the eukaryotic kingdom and promotes translation through physical and functional interactions with eukaryotic initiation factor (eIF) 4G and eIF4B.
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