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SAE2000

Sigma-Aldrich

SARS-COV-2-Spike-RBD epitope (370-394)

≥95% (HPLC), lyophilized powder

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Synonym(s):
COVID-19 epitope, Immune response epitope, SARS-COV-2 Spike protein peptide

Quality Level

form

lyophilized powder
solid

species reactivity

viral

UniProt accession no.

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

SARS coronavirus ... S(43740568)

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SAE2001SAB4200889SAB5700593
species reactivity

viral

species reactivity

viral

species reactivity

-

species reactivity

-

UniProt accession no.

P0DTC2

UniProt accession no.

P0DTC2

UniProt accession no.

P0DTC2

UniProt accession no.

P0DTC2

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Gene Information

SARS coronavirus ... S(43740568)

Gene Information

SARS coronavirus ... S(43740568)

Gene Information

-

Gene Information

SARS virus ... S(43740568)

target post-translational modification

unmodified

target post-translational modification

unmodified

target post-translational modification

unmodified

target post-translational modification

-

General description

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) or (2019-nCoV) is a novel coronavirus that emerged in December 2019 and since then has infected millions of people worldwide.1 The Spike protein (also known as S protein) is the most studied of the coronaviruses proteins, since it contains the Receptor-Binding-Domain (RBD) for the ligand on the host cell membrane (the ACE2 protein), and also has epitopes recognized by T and B cells

Specificity

SARS-COV-2-Spike-RBD epitope (370-394) is a synthetic peptide corresponding to the amino acid sequence of Spike RBD region (GeneID: QHD43416.1) in positions 370-394.Peptides derived from the SARS-COV-2-Spike-RBD protein can be recognized by anti-SARS-CoV-2-Spike protein antibodies.

Immunogen

S370-394: nsasfstfkcygvsptklndlcftn

Application

The peptide may be used in various immunochemical techniques including Immunoblotting and Elisa.

Biochem/physiol Actions

The Spike protein (also known as S protein) is a type I trimeric glycoprotein that is presented on the virion membrane, giving it the appearance of a crown. The protein has two subunits: S1, or bulb, that contains the RBD3-10; and S2, or stalk, responsible for the fusion of the virion with the host cell membrane. The main receptor for SARS-CoV and SARS-CoV-2 on the membrane of the target cells is the Angiotensin 2 Converting Enzyme (ACE2), a metallopeptidase present on the membrane of many cells, including type-I and -II pneumocytes, small intestine enterocytes, kidney proximal tubules cells, the endothelial cells of arteries and veins, and the arterial smooth muscle, among other tissues.15-18

Physical form

Supplied as a lyophilized powder.Purity: ≥95% (HPLC)

Storage and Stability

Store the product at −20 °C. After initial thawing, it is recommended to store the peptide in working aliquots at −20°C. Recommended thawing solution: Water.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Data presented is the available current product information and provided as-is. This product has not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Mining of epitopes on spike protein of SARS-CoV-2 from COVID-19 patients.
Bao-Zhong Zhang et al.
Cell research, 30(8), 702-704 (2020-07-03)
To Sing Fung et al.
Annual review of microbiology, 73, 529-557 (2019-06-22)
Human coronavirus (HCoV) infection causes respiratory diseases with mild to severe outcomes. In the last 15 years, we have witnessed the emergence of two zoonotic, highly pathogenic HCoVs: severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus
Jian Shang et al.
PLoS pathogens, 16(3), e1008392-e1008392 (2020-03-10)
Coronaviruses recognize a variety of receptors using different domains of their envelope-anchored spike protein. How these diverse receptor recognition patterns affect viral entry is unknown. Mouse hepatitis coronavirus (MHV) is the only known coronavirus that uses the N-terminal domain (NTD)
Daniel Wrapp et al.
Science (New York, N.Y.), 367(6483), 1260-1263 (2020-02-23)
The outbreak of a novel coronavirus (2019-nCoV) represents a pandemic threat that has been declared a public health emergency of international concern. The CoV spike (S) glycoprotein is a key target for vaccines, therapeutic antibodies, and diagnostics. To facilitate medical
Chunyan Yi et al.
Cellular & molecular immunology, 17(6), 621-630 (2020-05-18)
Coronavirus disease 2019 (COVID-19), caused by the novel human coronavirus SARS-CoV-2, is currently a major threat to public health worldwide. The viral spike protein binds the host receptor angiotensin-converting enzyme 2 (ACE2) via the receptor-binding domain (RBD), and thus is

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