SRP3003

Sigma-Aldrich

Activin A human

recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture

Synonym(s):
Activin beta-A, FRP, Inhibin beta-1
NACRES:
NA.32
Pricing and availability is not currently available.

biological source

human

recombinant

expressed in E. coli

assay

≥97% (HPLC)
≥97% (SDS-PAGE)

form

lyophilized

potency

≤2.0 ng/mL ED50

mol wt

26.0 kDa

packaging

pkg of 10 μg

application(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... INHBA(3624)
mouse ... Inhba(16323)
rat ... Inhba(29200)

General description

Activins (also referred to as inhibins) are generally dimers of three subunits: inhibin βA, inhibin βB, and inhibin α. Recombinant human Activin A (inhibin βA) is a 26kDa disulfide-linked homodimer of two bA chains, each containing 116 amino acid residues. The sequence of mature Activin A is completely homologous between human, mouse, and rat species.

Application

Activin A human has been used to study rRNA gene transcription during directed differentiation of human embryonic stem cells.
Activin A has been used in monolayer induction for endoderm (hepatocyte) differentiation.

Biochem/physiol Actions

Activin A (inhibin βA) is a TGF (transforming growth factor)-β family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. Elevated levels of Activin A in human colorectal tumors and in postmenopausal women have been implicated in colorectal and breast cancers, respectively. Activin A plays a central role in reproduction by regulating the levels of follicle stimulating hormone (FSH). It is involved in hematopoiesis, embryogenesis and osteogenesis. It exhibits both tumor suppressing and promoting effects. In underweight and overweight humans, high levels of Activin A in serum might be associated with the pathogenesis of asthma. The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-β antagonist, follistatin.

Sequence

GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Overexpression of activin A in stage IV colorectal cancer.
Wildi S, et al.
Gut, 49, 409-417 (2001)
Serum and tissue expression of activin a in postmenopausal women with breast cancer.
Reis FM, et al.
The Journal of Clinical Endocrinology and Metabolism, 87, 2277-2282 (2002)
Net increase in stimulatory input resulting from a decrease in inhibin B and an increase in activin A may contribute in part to the rise in follicular phase follicle-stimulating hormone of aging cycling women.
Reame NE, et al.
The Journal of Clinical Endocrinology and Metabolism, 83, 3302-3307 (1998)
Activin A is associated with asthma in underweight and overweight patients.
Zhang LL and Liu CT
Genetics and molecular research : GMR, 14, 440-452 (2015)
The Regulation of rRNA Gene Transcription during Directed Differentiation of Human Embryonic Stem Cells.
Woolnough JL, et al.
PLoS ONE, 11, e0157276-e0157276 (2016)

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