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  • LXR, a nuclear receptor that defines a distinct retinoid response pathway.

LXR, a nuclear receptor that defines a distinct retinoid response pathway.

Genes & development (1995-05-01)
P J Willy, K Umesono, E S Ong, R M Evans, R A Heyman, D J Mangelsdorf
ABSTRACT

We have identified a new retinoid response pathway through which 9-cis retinoic acid (9cRA) activates transcription in the presence of LXR alpha, a member of the nuclear receptor superfamily. LXR alpha shows a specific pattern of expression in visceral organs, thereby restricting the response to certain tissues. Retinoid trans-activation occurs selectively on a distinct response element termed an LXRE. Significantly, neither RXR homodimers nor RXR/RAR heterodimers are able to substitute for LXR alpha in mediating this retinoid response. We provide evidence that the retinoid response on the LXRE is the result of a unique interaction between LXR alpha and endogenous RXR, which, unlike in the RXR/RAR heterodimer, makes RXR competent to respond to retinoids. Thus, the interaction with LXR alpha shifts RXR from its role described previously as a silent, DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXREs.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
LXR β isoform, ligand binding domain (211-461), GST tagged human, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Sigma-Aldrich
LXR, β isoform, GST tagged human, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Sigma-Aldrich
LXR, α human, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Sigma-Aldrich
LXR, α, ligand binding domain, GST tagged human, recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Sigma-Aldrich
LXR, β human, recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Sigma-Aldrich
LXRα-ligand binding domain (215-667) human, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Sigma-Aldrich
LXR β-ligand binding domain (211-461) human, recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Sigma-Aldrich
LXR, α isoform, GST tagged human, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Sigma-Aldrich
LXR, α isoform, N-terminal region (1-182), GST tagged β human, recombinant, expressed in E. coli, ≥80% (SDS-PAGE)