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Diuretic screening in human urine by gas chromatography-mass spectrometry: use of a macroreticular acrylic copolymer for the efficient removal of the coextracted phase-transfer reagent after derivatization by direct extractive alkylation.

Journal of chromatography (1992-10-02)
A M Lisi, R Kazlauskas, G J Trout
ABSTRACT

A simple and efficient procedure has been developed for the derivatization of diuretic agents in human urine by direct extractive alkylation and their detection by gas chromatography-mass spectrometry. The procedure is an improvement over previous extractive alkylation methods because of the development of a simple clean-up step using a macroreticular acrylic copolymer (SM-7 resin) to remove the coextracted phase-transfer reagent from the organic phase after derivatization. With 1 ml of sample the method gives detection limits in the range 10-50 ng/ml for acetazolamide, probenecid, dichlorphenamide, hydroflumethiazide, furosemide, chlorthalidone, bumetanide, hydrochlorothiazide, quinethazone, bendroflumethiazide, metolazone and cyclopenthiazide.

MATERIALS
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Brand
Product Description

Supelco
Tetrahexylammonium hydrogensulfate, suitable for ion pair chromatography, LiChropur, ≥99.0% (T)
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Tetrahexylammonium hydrogensulfate, ≥98.0% (T)
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Tetrahexylammonium bromide, 99%
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Tetrahexylammonium chloride, 96%
Supelco
Tetrahexylammonium bromide, suitable for ion pair chromatography, LiChropur, ≥99.0% (AT)
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Tetrahexylammonium hydroxide solution, ~40% in H2O (T)
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Tetrahexylammonium iodide, ≥99.0% (AT)
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Tetrahexylammonium hydroxide solution, ~10% in methanol (T)

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