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  • [Extraction and analysis of chemical components of essential oil in Thymus vulgaris of tissue culture].

[Extraction and analysis of chemical components of essential oil in Thymus vulgaris of tissue culture].

Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (2012-03-01)
Xiao-Dong Li, Li Yang, Shi-Qian Xu, Jian-Guo Li, Zhi-Hui Cheng, Jian-Zhang Dang
ABSTRACT

To extract the essential oils from the Seedlings, the Aseptic Seedlings and the Tissue Culture Seedlings of Thymus vulgaris and analyze their chemical components and the relative contents. The essential oils were extracted by steam distillation, the chemical components and the relative contents were identified and analyzed by gas chromatography-mass spectrometry (GC/MS) and peak area normalization method. The main chemical components of essential oil in these three samples had no significant difference, they all contained the main components of essential oil in Thymus vulgaris: Thymol, Carvacrol, o-Cymene, gamma-Terpinene, Caryophyllene et al. and only had a slight difference in the relative content. This study provides important theoretical foundation and data reference for further study on production of essential oil in thyme by tissue culture technology.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
α-Terpinene, ≥89%, FCC, FG
Sigma-Aldrich
γ-Terpinene, ≥95%, FG
Sigma-Aldrich
γ-Terpinene, 97%
Sigma-Aldrich
α-Terpinene, 85%
Sigma-Aldrich
γ-Terpinene, ≥95.0% (GC)
Supelco
γ-Terpinene, analytical standard
Sigma-Aldrich
α-Terpinene, ≥95.0% (GC)
Sigma-Aldrich
γ-Terpinene, natural, 95%, FG