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Suborganelle sensing of mitochondrial cAMP-dependent protein kinase activity.

Journal of the American Chemical Society (2010-04-13)
Richard S Agnes, Finith Jernigan, Jennifer R Shell, Vyas Sharma, David S Lawrence
ABSTRACT

A fluorescent sensor of protein kinase activity has been developed and used to characterize the compartmentalized location of cAMP-dependent protein kinase activity in mitochondria. The sensor functions via a phosphorylation-induced release of a quencher from a peptide-based substrate, producing a 150-fold enhancement in fluorescence. The quenching phenomenon transpires via interaction of the quencher with Arg residues positioned on the peptide substrate. Although the cAMP-dependent protein kinase is known to be present in mitochondria, the relative amount of enzyme positioned in the major compartments (outer membrane, intermembrane space, and the matrix) of the organelle is unclear. The fluorescent sensor developed in this study was used to reveal the relative matrix/intermembrane space/outer membrane (85:6:9) distribution of PKA in bovine heart mitochondria.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Atto 425 NHS ester, BioReagent, suitable for fluorescence
Sigma-Aldrich
Atto 425
Sigma-Aldrich
Atto 425-Biotin, BioReagent, suitable for fluorescence, ≥95.0% (HPCE)
Sigma-Aldrich
Atto 425 maleimide, BioReagent, suitable for fluorescence, ≥70% (coupling to thiols)
Sigma-Aldrich
Atto 425 azide, suitable for fluorescence

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