Biofuels. Altered sterol composition renders yeast thermotolerant.

Science (New York, N.Y.) (2014-10-04)
Luis Caspeta, Yun Chen, Payam Ghiaci, Amir Feizi, Steen Buskov, Björn M Hallström, Dina Petranovic, Jens Nielsen
ABSTRACT

Ethanol production for use as a biofuel is mainly achieved through simultaneous saccharification and fermentation by yeast. Operating at ≥40°C would be beneficial in terms of increasing efficiency of the process and reducing costs, but yeast does not grow efficiently at those temperatures. We used adaptive laboratory evolution to select yeast strains with improved growth and ethanol production at ≥40°C. Sequencing of the whole genome, genome-wide gene expression, and metabolic-flux analyses revealed a change in sterol composition, from ergosterol to fecosterol, caused by mutations in the C-5 sterol desaturase gene, and increased expression of genes involved in sterol biosynthesis. Additionally, large chromosome III rearrangements and mutations in genes associated with DNA damage and respiration were found, but contributed less to the thermotolerant phenotype.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, for molecular biology
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, meets USP testing specifications
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%
Sigma-Aldrich
Reagent Alcohol, anhydrous, ≤0.003% water
Sigma-Aldrich
Reagent Alcohol, anhydrous, ≤0.005% water
Sigma-Aldrich
Ethanol, anhydrous, denatured
Sigma-Aldrich
Reagent Alcohol, reagent grade
Sigma-Aldrich
Ethyl alcohol, denatured, reagent grade
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, ACS reagent, ≥99.5%
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, HPLC/spectrophotometric grade
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, meets USP testing specifications
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, for molecular biology
Sigma-Aldrich
Ethanol, puriss. p.a., ACS reagent, prima fine spirit, without additive, F15 o1, ~96%
Supelco
Dehydrated Alcohol, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Ethanol solution, certified reference material, 2000 μg/mL in methanol
Supelco
Ethanol-100 (10 ampules/kit), 100 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Supelco
Ethanol-300, 300 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Supelco
Ethanol-10, 10 mg/dL in H2O, pack of 10 × 1.2 mL ampules, certified reference material, Cerilliant®
Sigma-Aldrich
Ergosterol, ≥75%
Sigma-Aldrich
Reagent Alcohol, suitable for HPLC
Supelco
Ethanol-80, 80 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Supelco
Ethanol standards 10% (v/v), 10 % (v/v) in H2O, analytical standard
Supelco
Ethanol-200 (10 ampules/kit), 200 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Supelco
Ethanol-50, 50 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Supelco
Ethanol-500, 500 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Supelco
Ethanol-20 (10 ampules/kit), 20 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Supelco
Ethanol-400 (10 ampules/kit), 400 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Supelco
Ethanol-25, 25 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Supelco
Ethanol Calibration Kit, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.